A Revised Spiralian Homeobox Gene Classification Incorporating New Polychaete Transcriptomes Reveals a Diverse TALE Class and a Divergent Hox Gene.
A Revised Spiralian Homeobox Gene Classification Incorporating New Polychaete Transcriptomes Reveals a Diverse TALE Class and a Divergent Hox Gene.
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DOI：
10.1093/gbe/evy144
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发表时间：
2018-09-01
影响因子：
3.3
通讯作者：
Ferrier DEK
中科院分区：
文献类型：
--
作者：
Barton-Owen TB;Szabó R;Somorjai IML;Ferrier DEK
后生动物再生机制和能力的多样性对进化生物学提出了有趣的挑战，影响着新兴的再生医学领域。广泛的分类学采样对于提高我们对再生的理解至关重要，传统模式生物之外的研究已被证明提供了非常丰富的信息。在历史上未被充分研究的螺旋虫中，环节动物拥有令人印象深刻的各种易处理的再生系统。蛇形多毛类龙骨虫 Spirobranchus（以前称为 Pomatoceros）lamarcki 的头部附属器（盖）的生物聚合、无芽基再生就是这样的系统之一。为了分析潜在的调控机制，我们对鳃盖再生转录组的同源框基因内容进行了分类。由于检索了几个难以分类的同源盒序列，我们对广泛选择的冠轮动物基因组的 TALE 和 PRD 类同源盒基因内容进行了广泛的搜索和系统发育分析。这些分析有助于我们加深对这些最近发现的同源盒基因辐射的多样性、分类范围、快速进化和根本灵活性的了解。我们对以前的命名系统的扩展和集成有助于澄清它们神秘的同源关系。我们还描述了一个不寻常的分歧 S. lamarcki Antp 基因、一个以前未分类的冠轮动物孤儿基因家族 (Lopx) 和一些新的 Nk 类孤儿基因。许多谱系和进化枝限制的同源盒基因在拉马克链霉菌再生中的表达和潜在参与提供了再生机制多样性的例子，并显着提高了我们对同源盒基因进化的理解。
The diversity of mechanisms and capacity for regeneration across the Metazoa present an intriguing challenge in evolutionary biology, impacting on the burgeoning field of regenerative medicine. Broad taxonomic sampling is essential to improve our understanding of regeneration, and studies outside of the traditional model organisms have proved extremely informative. Within the historically understudied Spiralia, the Annelida have an impressive variety of tractable regenerative systems. The biomeralizing, blastema-less regeneration of the head appendage (operculum) of the serpulid polychaete keelworm Spirobranchus (formerly Pomatoceros) lamarcki is one such system. To profile potential regulatory mechanisms, we classified the homeobox gene content of opercular regeneration transcriptomes. As a result of retrieving several difficult-to-classify homeobox sequences, we performed an extensive search and phylogenetic analysis of the TALE and PRD-class homeobox gene content of a broad selection of lophotrochozoan genomes. These analyses contribute to our increasing understanding of the diversity, taxonomic extent, rapid evolution, and radical flexibility of these recently discovered homeobox gene radiations. Our expansion and integration of previous nomenclature systems helps to clarify their cryptic orthology. We also describe an unusual divergent S. lamarcki Antp gene, a previously unclassified lophotrochozoan orphan gene family (Lopx), and a number of novel Nk class orphan genes. The expression and potential involvement of many of these lineage- and clade-restricted homeobox genes in S. lamarcki operculum regeneration provides an example of diversity in regenerative mechanisms, as well as significantly improving our understanding of homeobox gene evolution.
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影响因子：
3.7
作者：
Frobius, Andreas C.;Matus, David Q.;Seaver, Elaine C.
通讯作者：
Seaver, Elaine C.
影响因子：
--
作者：
Gharbaran, Rajendra;Alvarado, Susana;Aisemberg, Gabriel O.
通讯作者：
Aisemberg, Gabriel O.
影响因子：
0.7
作者：
Balavoine, Guillaume
通讯作者：
Balavoine, Guillaume
影响因子：
3.7
作者：
de Jong DM;Seaver EC
通讯作者：
Seaver EC
影响因子：
1.6
作者：
Bok, Michael J.;Porter, Megan L.;Nilsson, Dan-Eric
通讯作者：
Nilsson, Dan-Eric