The receptor-like kinase SERK3/BAK1 is required for basal resistance against the late blight pathogen phytophthora infestans in Nicotiana benthamiana.
The receptor-like kinase SERK3/BAK1 is required for basal resistance against the late blight pathogen phytophthora infestans in Nicotiana benthamiana.
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DOI：
10.1371/journal.pone.0016608
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发表时间：
2011-01-27
期刊：
影响因子：
3.7
通讯作者：
Schornack S
中科院分区：
文献类型：
--
作者：
Chaparro-Garcia A;Wilkinson RC;Gimenez-Ibanez S;Findlay K;Coffey MD;Zipfel C;Rathjen JP;Kamoun S;Schornack S
丝状卵菌植物病原菌疫霉（Phytophthora infestans）对茄科作物马铃薯和番茄等植物造成晚疫病，这是一种重要的经济病害。相关植物本菌烟（Nicotiana benthamiana）是研究植物与病原菌相互作用的模型系统，本菌烟（Nicotiana benthamiana）对疫霉菌的敏感性从敏感到抗性不等。对于识别保守病原体相关分子模式（PAMPs）的膜受体介导的植物基础免疫在多大程度上促进了病原菌的抗性，我们知之甚少。我们发现不同种类的疫霉对benthamiana具有不同程度的毒力，从无毒（不相容相互作用）到中等毒力到完全侵袭性。富含亮氨酸的重复受体样激酶（LRR-RLK） BAK1/SERK3是拟南芥和拟南芥中pamp触发免疫（PTI）的主要调节剂。基于与拟南芥基因的序列相似性，我们从benthamiana中克隆了两个NbSerk3同源物NbSerk3A和NbSerk3B。对NbSerk3基因沉默的benthamiana植物对病原菌感染的易感性明显增强，但对致病性疫霉（Phytophthora mirabilis）的抗性没有改变，后者是病原菌的姐妹种，专门针对不同的寄主植物。此外，NbSerk3的沉默减少了由INF1触发的细胞死亡反应，INF1是一种分泌的具有PAMPs特征的P. infestans蛋白。我们证明了benthamiana NbSERK3显著有助于对P. infestans的抗性，并调节由P. infestans PAMP蛋白INF1触发的免疫反应。在未来，与NbSERK3A和/或NbSERK3B相关的新型表面受体的鉴定应该会导致介导卵菌PAMPs识别的新受体的鉴定，如INF1。
The filamentous oomycete plant pathogen Phytophthora infestans causes late blight, an economically important disease, on members of the nightshade family (Solanaceae), such as the crop plants potato and tomato. The related plant Nicotiana benthamiana is a model system to study plant-pathogen interactions, and the susceptibility of N. benthamiana to Phytophthora species varies from susceptible to resistant. Little is known about the extent to which plant basal immunity, mediated by membrane receptors that recognise conserved pathogen-associated molecular patterns (PAMPs), contributes to P. infestans resistance. We found that different species of Phytophthora have varying degrees of virulence on N. benthamiana ranging from avirulence (incompatible interaction) to moderate virulence through to full aggressiveness. The leucine-rich repeat receptor-like kinase (LRR-RLK) BAK1/SERK3 is a major modulator of PAMP-triggered immunity (PTI) in Arabidopsis thaliana and N. benthamiana. We cloned two NbSerk3 homologs, NbSerk3A and NbSerk3B, from N. benthamiana based on sequence similarity to the A. thaliana gene. N. benthamiana plants silenced for NbSerk3 showed markedly enhanced susceptibility to P. infestans infection but were not altered in resistance to Phytophthora mirabilis, a sister species of P. infestans that specializes on a different host plant. Furthermore, silencing of NbSerk3 reduced the cell death response triggered by the INF1, a secreted P. infestans protein with features of PAMPs. We demonstrated that N. benthamiana NbSERK3 significantly contributes to resistance to P. infestans and regulates the immune responses triggered by the P. infestans PAMP protein INF1. In the future, the identification of novel surface receptors that associate with NbSERK3A and/or NbSERK3B should lead to the identification of new receptors that mediate recognition of oomycete PAMPs, such as INF1.
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