In vitro GRANULOMA FORMATION BY SPLEEN CELLS TREATED WITH PSORALEN PLUS LONG‐WAVE ULTRAVIOLET RADIATION
In vitro GRANULOMA FORMATION BY SPLEEN CELLS TREATED WITH PSORALEN PLUS LONG‐WAVE ULTRAVIOLET RADIATION
复制标题
用补骨脂素加长波紫外线辐射处理的脾细胞的体外肉芽肿形成
DOI：
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发表时间：
1993
影响因子：
3.3
通讯作者：
S. Imamura
中科院分区：
文献类型：
--
作者：
H. Okamoto;Zaipei Guo;S. Imamura
摘要：用人造微粒、葡聚糖珠培养小鼠脾细胞诱导体外肉芽肿。在 0.5 μg/mL 8-甲氧基补骨脂存在的情况下，UVA 辐射（0.2-2.0 J/cm2 以 UVA 剂量依赖性方式抑制肉芽肿形成素。根据台盼蓝排斥法评估，PUVA 的剂量不影响细胞活力。0.5 J/cm2 PUVA 处理的细胞中肉芽肿形成的时间过程与正常脾细胞相似，每天具有最小肉芽肿指数3 的培养物，尽管在 PUVA 处理的贴壁细胞中观察到 49-63% 的肉芽肿形成抑制，即使它们与正常的非贴壁细胞一起培养，这些数据表明 PUVA 改变巨噬细胞，导致体外肉芽肿形成的抑制。
Abstract— In vitro gransulomas ar induced by culturing murine spleen cells with artificial microparticles, dextran beads. In the presence of 0.5 μg/mL 8‐methoxypsorlen, UVA radiation (0.2‐2.0 J/cm2 suppressed granuloma formatin in a UVA dose‐ dependent manner. The dose of PUVA did not affect the cell viability as assessed by trypan blue exclusion. The time course of granuloma formation in 0.5 J/cm2 PUVA‐treated cells was similar to that of normal spleen cells, with a miximum granuloma index at day 3 of culture, althought a49–63% suppression of granuloma formation was observed in PUVA‐treated, adherent cells even when they were cultured with normal nonadherent cells. These data suggests that PUVA alters macrophages, resulting in the suppression of granuloma formation in vitro
在接受长期补骨脂素/紫外线 A 辐射 (PUVA) 治疗的银屑病患者中，单克隆抗体识别的循环辅助诱导 T 细胞比例减少。
DOI：
10.1111/1523-1747.ep12500058
发表时间：
1982
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Moscicki,RA;Morison,WL;Parrish,JA;Bloch,KJ;Colvin,RB
通讯作者：
Colvin,RB