Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae).
Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae).
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白芨属（兰科）根内生真菌群落和碳氮稳定同位素模式存在差异
DOI：
10.3390/jof7020069
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发表时间：
2021-01-20
期刊：
影响因子：
--
通讯作者：
Huang W
中科院分区：
文献类型：
--
作者：
Zeng X;Ni Z;Diao H;Jiang K;Hu C;Shao L;Huang W
白芨属兰花是亚洲著名的观赏植物和传统药物来源，其整个生命周期依赖于与根部内生真菌的共生关系。然而，人们对它们的真菌伙伴、感染模式和碳增益途径知之甚少。我们研究了三个白及物种不同器官中的碳和氮稳定同位素模式，确定了根内生真菌群落组成，并确定了菌根定植率。三个白及属属于一个多系类群，属于不同的营养模式，如腐养型、病养型和共生型；然而，白芨属的优势种及其丰度有所不同。白芨菌根感染率也存在差异，其中白芨（65%±25%）显着高于白芨（35%±16%）和黄白芩（22%±13%）。与周围的自养植物相比，所有白芨属植物。 B. striata 在 13C 中显着富集，其水平显着高于其他两种白芨物种。在不同器官中，三个物种的茎具有较高的 δ13C 值，而叶和花具有较高的 δ15N 和总氮含量值。我们的结果表明，白及及其根内生真菌的共生关系并不严格特定。尽管菌根感染率差异很大，但这三个白及属物种具有相同的感染模式，即菌丝通过途径细胞穿透皮层细胞。不同白及属植物器官之间的碳分配策略不同。这些发现为兰花的生态适应提供了新的见解，并将有助于白芨种质的保护和可持续利用。
Orchids of the genus Bletilla are well-known ornamental plants and sources of traditional medicine in Asia that rely on the symbiotic relationship with root endophytic fungi throughout their whole life cycle. However, little is known about their fungal partners, infection pattern, and pathways of carbon gain. We investigated carbon and nitrogen stable isotope patterns in different organs of three Bletilla species, identified the root endophytic fungal community composition, and determined mycorrhizal colonization rates. The three Bletilla species were comprised by a polyphyletic group which belongs to different trophic modes, such as saprotroph, pathotroph, and symbiotroph; however, the dominant species and their abundances varied among Bletilla spp. Mycorrhizal infection rates also varied among Bletilla species, with B. striata (65% ± 25%) being significantly higher than those of B. formosana (35% ± 16%) and B. ochracea (22% ± 13%). Compared with surrounding autotrophic plants, all Bletilla spp. were significantly enriched in 13C with B. striata to a significantly higher level than other two Bletilla species. Among different organs, stems had higher δ13C values, while leaves and flowers had higher δ15N and total N content values across all three species. Our results indicate that the symbiotic relationship of Bletilla and its root endophytic fungi is not strictly specific. Although mycorrhizal infection rates were highly variable, the three Bletilla species had the same infection pattern with hyphae penetrating the cortex cell by the pathway cell. Different Bletilla species have different strategies for C allocation among plant organs. These findings provide new insights into the ecological adaptation of orchids and will contribute to Bletilla germplasm conservation and sustainable utilization.
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影响因子：
3.2
作者：
Farh, Mohamed El-Agamy;Kim, Yeon-Ju;Yang, Deok-Chun
通讯作者：
Yang, Deok-Chun
DOI：
10.1098/rspb.2004.2807
发表时间：
2004-09-07
影响因子：
4.7
作者：
Bidartondo, MI;Burghardt, B;Read, DJ
通讯作者：
Read, DJ
DOI：
10.1080/10256019308046133
发表时间：
1993-01-01
期刊：
ISOTOPENPRAXIS
影响因子：
--
作者：
GEBAUER, G;DIETRICH, P
通讯作者：
DIETRICH, P
影响因子：
9.4
作者：
Gebauer, G;Meyer, M
通讯作者：
Meyer, M
影响因子：
7.4
作者：
GLEIXNER, G;DANIER, HJ;SCHMIDT, HL
通讯作者：
SCHMIDT, HL