Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice.
Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice.
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DOI：
10.3389/fncir.2010.00009
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发表时间：
2010
影响因子：
3.5
通讯作者：
Hasan MT
中科院分区：
文献类型：
--
作者：
Lütcke H;Murayama M;Hahn T;Margolis DJ;Astori S;Zum Alten Borgloh SM;Göbel W;Yang Y;Tang W;Kügler S;Sprengel R;Nagai T;Miyawaki A;Larkum ME;Helmchen F;Hasan MT
荧光钙（Ca 2+）指示蛋白（FCIPs）是活体动物细胞活性功能成像的有前途的工具。然而，由于表达水平、动态范围和报告动作电位的灵敏度的限制，它们仍然没有达到其用于神经元活性的体内成像的全部潜力。在这里，我们报告说，病毒表达的比率Ca 2+传感器黄色cameleon 3.60（YC3.60）在小鼠桶皮质的锥体神经元，使在体内测量的神经元活动的高动态范围和灵敏度在多个空间尺度。通过结合体外和体内的细胞外记录和双光子成像，我们证明了YC 3.60可以分辨单个动作电位（AP）诱发的Ca 2+瞬变，并可靠地报告AP的爆发，其饱和度可以忽略不计。自发和晶须诱发的钙瞬变检测在个别顶端树突和胞体以及在当地的神经元群体。此外，使用宽场成像或光纤进行的批量测量显示，在桶场的大面积区域中存在感官诱发的YC 3.60信号。特别是光纤记录能够在清醒的自由移动的小鼠中进行测量，并揭示了复杂的Ca 2+动力学，可能反映了不同的行为相关的大脑状态。病毒表达的YC3.60 -结合各种光学技术-从而打开了许多机会的动物行为的神经基础的功能研究，从树突的水平，本地和大规模的神经元群体。
Fluorescent calcium (Ca2+) indicator proteins (FCIPs) are promising tools for functional imaging of cellular activity in living animals. However, they have still not reached their full potential for in vivo imaging of neuronal activity due to limitations in expression levels, dynamic range, and sensitivity for reporting action potentials. Here, we report that viral expression of the ratiometric Ca2+ sensor yellow cameleon 3.60 (YC3.60) in pyramidal neurons of mouse barrel cortex enables in vivo measurement of neuronal activity with high dynamic range and sensitivity across multiple spatial scales. By combining juxtacellular recordings and two-photon imaging in vitro and in vivo, we demonstrate that YC3.60 can resolve single action potential (AP)-evoked Ca2+ transients and reliably reports bursts of APs with negligible saturation. Spontaneous and whisker-evoked Ca2+ transients were detected in individual apical dendrites and somata as well as in local neuronal populations. Moreover, bulk measurements using wide-field imaging or fiber-optics revealed sensory-evoked YC3.60 signals in large areas of the barrel field. Fiber-optic recordings in particular enabled measurements in awake, freely moving mice and revealed complex Ca2+ dynamics, possibly reflecting different behavior-related brain states. Viral expression of YC3.60 – in combination with various optical techniques – thus opens a multitude of opportunities for functional studies of the neural basis of animal behavior, from dendrites to the levels of local and large-scale neuronal populations.
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影响因子：
14.8
作者：
Garaschuk, Olga;Milos, Ruxandra-Iulia;Konnerth, Arthur
通讯作者：
Konnerth, Arthur
影响因子：
12.4
作者：
Mastakov, MY;Baer, K;During, MJ
通讯作者：
During, MJ
影响因子：
25
作者：
Madisen L;Zwingman TA;Sunkin SM;Oh SW;Zariwala HA;Gu H;Ng LL;Palmiter RD;Hawrylycz MJ;Jones AR;Lein ES;Zeng H
通讯作者：
Zeng H
DOI：
10.1007/s11068-008-9029-4
发表时间：
2008-08-01
期刊：
BRAIN CELL BIOLOGY
影响因子：
--
作者：
Hires, S. Andrew;Tian, Lin;Looger, Loren L.
通讯作者：
Looger, Loren L.
影响因子：
3.7
作者：
Mao T;O'Connor DH;Scheuss V;Nakai J;Svoboda K
通讯作者：
Svoboda K