Effect of skimmed pasteurized milk and Hank's balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells.
Effect of skimmed pasteurized milk and Hank's balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells.
复制标题
脱脂巴氏奶和汉克平衡盐溶液对人牙周膜干细胞活力和成骨分化的影响。
DOI：
10.1111/edt.12007
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
L. Ge
中科院分区：
文献类型：
--
作者：
Wen;Yuming Zhao;Xiao;W. Jia;L. Ge
背景/目标
本研究的目的是比较脱脂巴氏杀菌奶和Hank‘s平衡盐液对体外培养的人牙周膜干细胞活力和成骨分化潜能的影响。
材料和方法
从健康第三磨牙中提取人牙周膜干细胞，在脱脂巴氏牛奶和Hank‘s平衡盐液中室温保存1、2、4h，检测细胞活力和成骨分化潜能。
结果
在4h时，脱脂巴氏杀菌乳对细胞活力的影响显著高于HBSS(P<；0.05)，而在2和4h时，脱脂巴氏杀菌乳中细胞的矿化程度显著高于HBSS(P<；0.05)。
结论
脱脂巴氏杀菌乳比Hank‘s平衡盐液更能维持体外培养的PDLSCs的活性和成骨分化潜能。
BACKGROUND/AIM
The purpose of this study was to compare the effect of skimmed pasteurized milk and Hank's balanced salt solution on the viability and osteogenic differentiation potential of the human periodontal ligament stem cells at room temperature in vitro.
MATERIAL AND METHODS
Human periodontal ligament stem cells were obtained from extracted healthy third molars and conserved in skimmed pasteurized milk and Hank's balanced salt solution for 1, 2, and 4 h at room temperature to detect the viability of the cells and their osteogenic differentiation potential.
RESULTS
The efficacy of skimmed pasteurized milk on cell viability at 4 h was significantly higher than that of HBSS (P < 0.05), and cells stored in skimmed pasteurized milk showed significantly higher levels of mineralization than those in HBSS at 2 and 4 h (P < 0.05).
CONCLUSIONS
Skimmed pasteurized milk was more effective than Hank's balanced salt solution in maintaining the viability and osteogenic differentiation potential of PDLSCs at room temperature in vitro.
DOI：
10.1073/pnas.240309797
发表时间：
2000-12-05
影响因子：
11.1
作者：
Gronthos, S;Mankani, M;Shi, S
通讯作者：
Shi, S