Filamentation initiated by Cas2 and its association with the acquisition process in cells
Filamentation initiated by Cas2 and its association with the acquisition process in cells
复制标题
Cas2 启动的丝状结构及其与细胞内获取过程的关联
DOI：
10.1038/s41368-019-0063-0
复制
发表时间：
2019-09
影响因子：
14.9
通讯作者：
Li Chen
中科院分区：
文献类型：
--
作者：
Lei Wang;Xin Yu;Mengjie Li;Guiqin Sun;Lin Zou;Tiansheng Li;Linlin Hou;Yameng Guo;Danfeng Shen;DiQu;Xunjia Chen;Li Chen
Cas1和Cas2介导的新间隔区获得是细菌获得性免疫的重要过程。这一过程对口腔微生物生态和口腔健康至关重要。虽然间隔区获取的分子机制是已知的，但如果该过程与细菌的形态学变化相关，则从未建立。在这项研究中，我们证明了一种新的Cas2诱导的大肠杆菌表达表型。大肠杆菌中表达Cas1蛋白。在Cas2的羧基末端的30个氨基酸基序对于该功能是必需的。通过成像分析，我们提供的证据表明，Cas诱导的表达是一个步骤，加上新的间隔区收购期间，丝的特点是多倍体与不对称的细胞分裂。这项工作可能为研究口腔健康的适应性免疫反应和微生物平衡开辟新的机会。
Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity. The process is critical for the ecology of the oral microflora and oral health. Although molecular mechanisms for spacer acquisition are known, it has never been established if this process is associated with the morphological changes of bacteria. In this study, we demonstrated a novel Cas2-induced filamentation phenotype inE. colithat was regulated by co-expression of the Cas1 protein. A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function. By imaging analysis, we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division. This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.
登录
查看更多内容
影响因子：
3.2
作者：
L. Pine;C. J. Boone
通讯作者：
L. Pine;C. J. Boone
影响因子：
64.5
作者：
Dillard KE;Brown MW;Johnson NV;Xiao Y;Dolan A;Hernandez E;Dahlhauser SD;Kim Y;Myler LR;Anslyn EV;Ke A;Finkelstein IJ
通讯作者：
Finkelstein IJ
影响因子：
64.8
作者：
Levy A;Goren MG;Yosef I;Auster O;Manor M;Amitai G;Edgar R;Qimron U;Sorek R
通讯作者：
Sorek R
脑膜脓毒症伊丽莎白金菌核心岩藻糖苷酶的鉴定和表征
影响因子：
4.8
作者：
Li，Tiansheng;Li，Mengjie;Chen，Li
通讯作者：
Chen，Li
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle