Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells.
Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells.
复制标题
工程细胞材料界面，用于人类多能干细胞长期扩张。
DOI：
10.1016/j.biomaterials.2012.10.020
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发表时间：
2013-01
期刊：
影响因子：
14
通讯作者：
Varghese S
中科院分区：
文献类型：
--
作者：
Chang CW;Hwang Y;Brafman D;Hagan T;Phung C;Varghese S
支持人类多能干细胞(HPSCs)长期扩增的低成本、可扩展的合成基质有许多应用，从药物筛选平台到再生医学。在这里，我们报告了一种基于水凝胶的基质的开发，该基质含有合成的类似肝素的部分，支持hPSC(≥20代)在化学定义的介质中的长期扩张。在此合成基质上扩增的hPSCs保持了其特有的形态、集落形成能力、核型稳定性和分化潜能。我们还以合成的基质为平台，研究了细胞外环境的各种物理化学性质对hPSCs的黏附、生长和自我更新的影响。观察到的细胞反应可以用基质界面介导的细胞外基质蛋白、生长因子和其他细胞分泌因子的结合来解释，这为hPSCs的自我更新创造了一个有益的微环境。这些合成基质由现成的成分组成，很容易合成，为阐明控制干细胞命运的分子机制提供了理想的工具。
Cost-effective and scalable synthetic matrices that support long-term expansion of human pluripotent stem cells (hPSCs) have many applications, ranging from drug screening platforms to regenerative medicine. Here, we report the development of a hydrogel-based matrix containing synthetic heparin-mimicking moieties that supports the long-term expansion of hPSCs (≥20 passages) in a chemically defined medium. HPSCs expanded on this synthetic matrix maintained their characteristic morphology, colony forming ability, karyotypic stability, and differentiation potential. We also used the synthetic matrix as a platform to investigate the effects of various physicochemical properties of the extracellular environment on the adhesion, growth, and self-renewal of hPSCs. The observed cellular responses can be explained in terms of matrix interface-mediated binding of extracellular matrix proteins, growth factors, and other cell secreted factors, which create an instructive microenvironment to support self-renewal of hPSCs. These synthetic matrices, which comprise of “off-the-shelf” components and are easy to synthesize, provide an ideal tool to elucidate the molecular mechanisms that control stem cell fate.
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影响因子：
6.2
作者：
Sangaj N;Kyriakakis P;Yang D;Chang CW;Arya G;Varghese S
通讯作者：
Varghese S
影响因子：
4.1
作者：
Kolhar, Poornima;Kotamraju, Venkata Ramana;Ruoslahti, Erkki
通讯作者：
Ruoslahti, Erkki
DOI：
10.1073/pnas.1114854108
发表时间：
2011-11-15
影响因子：
11.1
作者：
Saha, Krishanu;Mei, Ying;Jaenisch, Rudolf
通讯作者：
Jaenisch, Rudolf
影响因子：
5.2
作者：
Hayashi, Yohei;Furue, Miho Kusuda;Asashima, Makoto
通讯作者：
Asashima, Makoto
DOI：
10.1073/pnas.1103100109
发表时间：
2012-01-17
影响因子：
11.1
作者：
Przybyla, Laralynne M.;Voldman, Joel
通讯作者：
Voldman, Joel