Hierarchical organization and early hematopoietic specification of the developing HSC lineage in the AGM region.
Hierarchical organization and early hematopoietic specification of the developing HSC lineage in the AGM region.
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AGM地区发展中HSC谱系的分层组织和早期造血规范。
DOI：
10.1084/jem.20102419
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发表时间：
2011-06-06
期刊：
影响因子：
--
通讯作者：
Medvinsky A
中科院分区：
文献类型：
--
作者：
Rybtsov S;Sobiesiak M;Taoudi S;Souilhol C;Senserrich J;Liakhovitskaia A;Ivanovs A;Frampton J;Zhao S;Medvinsky A
CD45 阴性的前 HSC 群体在胚胎 AGM 区域发育成最终的 HSC。主动脉-性腺-中肾区域在小鼠胚胎发生过程中的造血干细胞（HSC）发育中发挥着重要作用。血管内皮钙粘蛋白+ CD45+ (VE-cad+CD45+) 群体包含主要类型的未成熟前HSC，能够发育成长期再增殖的最终HSC。在这项研究中，我们开发了一种新的共聚集培养系统，该系统支持新的 CD45 阴性 (VE-cad+CD45−CD41+) 前 HSC 群体成熟为最终的 HSC。这些前 HSC 的出现先于 VE-cad+CD45+ 前 HSC（此处分别称为 I 型和 II 型前 HSC）的发育，从而在发育中的 HSC 谱系中建立了分层方向性。通过标记背主动脉的管腔表面，我们发现 I 型和 II 型前 HSC 广泛分布于主动脉内皮层和内皮下层，而成熟的定型 HSC 则定位于主动脉内皮层。与 CD41 在前 HSC 中的表达一致，体内 CD41-Cre 介导的基因标记发生在胚胎前 HSC 中，并在成年动物的所有淋巴骨髓谱系中持续存在。
A CD45-negative population of pre-HSCs develops into definitive HSCs in the AGM region of the embryo. The aorta-gonad-mesonephros region plays an important role in hematopoietic stem cell (HSC) development during mouse embryogenesis. The vascular endothelial cadherin+ CD45+ (VE-cad+CD45+) population contains the major type of immature pre-HSCs capable of developing into long-term repopulating definitive HSCs. In this study, we developed a new coaggregation culture system, which supports maturation of a novel population of CD45-negative (VE-cad+CD45−CD41+) pre-HSCs into definitive HSCs. The appearance of these pre-HSCs precedes development of the VE-cad+CD45+ pre-HSCs (termed here type I and type II pre-HSCs, respectively), thus establishing a hierarchical directionality in the developing HSC lineage. By labeling the luminal surface of the dorsal aorta, we show that both type I and type II pre-HSCs are distributed broadly within the endothelial and subendothelial aortic layers, in contrast to mature definitive HSCs which localize to the aortic endothelial layer. In agreement with expression of CD41 in pre-HSCs, in vivo CD41-Cre–mediated genetic tagging occurs in embryonic pre-HSCs and persists in all lymphomyeloid lineages of the adult animal.
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影响因子：
64.8
作者：
Chen, Michael J.;Yokomizo, Tomomasa;Zeigler, Brandon M.;Dzierzak, Elaine;Speck, Nancy A.
通讯作者：
Speck, Nancy A.
影响因子：
4
作者：
Li, WM;Ferkowicz, MJ;Yoder, MC
通讯作者：
Yoder, MC
影响因子：
20.3
作者：
McKinney-Freeman, Shannon L.;Naveiras, Olaia;Daley, George Q.
通讯作者：
Daley, George Q.
影响因子：
20.3
作者：
Mikkola, HKA;Fujiwara, Y;Orkin, SH
通讯作者：
Orkin, SH
DOI：
10.1073/pnas.0402270102
发表时间：
2005-01-04
影响因子：
11.1
作者：
Bertrand, JY;Giroux, S;Cumano, A
通讯作者：
Cumano, A