In vitro spermatogenesis: A century-long research journey, still half way around.
In vitro spermatogenesis: A century-long research journey, still half way around.
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DOI：
10.1002/rmb2.12225
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发表时间：
2018-10
影响因子：
3.4
通讯作者：
Ogawa T
中科院分区：
文献类型：
--
作者：
Komeya M;Sato T;Ogawa T
精子发生是体内最复杂的细胞分化过程之一。研究人员努力寻找和开发一种可以在体外支持这一过程的微环境条件。这种努力可以追溯到一个世纪前，并且仍在继续。筛选了体外精子发生及相关工作的报告，并根据所使用的方法分为四类；器官培养、肾小管培养、细胞培养和三维细胞培养方法。每份报告均由十多年来一直致力于器官培养方法体外精子发生的作者从当前角度进行了严格审查。器官培养法历史最悠久，也是最成功的方法，由精原干细胞产生可育的小鼠精子。培养基的配方是一个关键因素，最重要的是血清衍生物质。然而，血清中诱导和支持培养组织中精子发生的因子仍有待鉴定。此外，小鼠精子发生的成功尚未应用于其他动物。回顾细胞培养方法的历史，我们发现支持细胞作为饲养细胞发挥着重要作用。然而，即使对于支持细胞，生精发育也仅限于生精的一小部分，例如减数分裂前期的一段时期。类器官或三维培养技术的最新发展前景广阔，但仍需要进一步完善。体外精子发生的研究在上个世纪取得了显着进展。然而，我们需要做更多的工作来建立一个培养系统，可以诱导和维持许多（如果不是全部）哺乳动物物种的完整精子发生。
Spermatogenesis is one of the most complicated cellular differentiation processes in a body. Researchers struggled to find and develop a micro‐environmental condition that can support the process in vitro. Such endeavors can be traced back to a century ago and are yet continuing. Reports on in vitro spermatogenesis and related works were selected and classified into four categories based on the method used; organ culture, tubule culture, cell culture, and 3‐dimensional cell culture methods. Each report was critically reviewed from the present point of view by authors who have been working on in vitro spermatogenesis with organ culture method over a decade. The organ culture method has the longest history and is the most successful method, which produced fertile mouse sperm from spermatogonial stem cells. Formulation of the medium was a key factor, most importantly serum‐derived substances. However, factors in the serum that induce and support spermatogenesis in the cultured tissue remain to be identified. In addition, the success of mouse spermatogenesis is yet to be applied to other animals. On looking into the history of cell culture method, it became clear that Sertoli cells as feeder cells play an important role. Even with Sertoli cells, however, spermatogenic development has been limited to small parts of spermatogenesis, a segmented period of meiotic prophase for instance. Recent developments of organoid or 3‐dimensional culture techniques are promising but they still need further refinements. The study of in vitro spermatogenesis progressed significantly over the last century. We need more work, however, to establish a culture system that can induce and maintain complete spermatogenesis of many if not all mammalian species.
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影响因子：
4.6
作者：
Komeya M;Hayashi K;Nakamura H;Yamanaka H;Sanjo H;Kojima K;Sato T;Yao M;Kimura H;Fujii T;Ogawa T
通讯作者：
Ogawa T
影响因子：
4.6
作者：
Komeya M;Kimura H;Nakamura H;Yokonishi T;Sato T;Kojima K;Hayashi K;Katagiri K;Yamanaka H;Sanjo H;Yao M;Kamimura S;Inoue K;Ogonuki N;Ogura A;Fujii T;Ogawa T
通讯作者：
Ogawa T
影响因子：
3.6
作者：
Hue, D;Staub, C;Durand, P
通讯作者：
Durand, P
影响因子：
2.9
作者：
Abu Elhija, Mahmoud;Lunenfeld, Eitan;Huleihel, Mahmoud
通讯作者：
Huleihel, Mahmoud
影响因子：
--
作者：
Bascom, KF;Osterud, HL
通讯作者：
Osterud, HL