Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
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DOI：
10.1038/s41598-017-15799-3
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发表时间：
2017-11-13
影响因子：
4.6
通讯作者：
Ogawa T
中科院分区：
文献类型：
--
作者：
Komeya M;Hayashi K;Nakamura H;Yamanaka H;Sanjo H;Kojima K;Sato T;Yao M;Kimura H;Fujii T;Ogawa T
三维聚集和器官培养方法是重建体外活体细胞现象的关键。此前，我们使用常规的气液相间器官培养方法诱导小鼠完全生精。在加入微流控系统后，我们实现了精子生成效率和持续时间的显著提高。然而，阻碍微流体普及的主要缺点之一是使用动力泵来产生介质流。在这项研究中，我们制造了一种无泵微流控装置，该装置使用静液压和阻力回路来促进缓慢、持久的介质流动。在三个月的培养中，无泵和泵驱动的装置在诱导和维持精子发生方面的结果没有差异。相应地，与常规方法相比，无泵装置能更好地维持精原细胞的数量。这些结果显示了使用微流控系统进行器官培养实验的优势。我们的无泵装置可以应用于各种其他组织和器官，并可能从整体上革命器官培养方法。
Three-dimensional aggregation and organ culture methods are critical for recreating in vivo cellular phenomena outside the body. Previously, we used the conventional gas liquid interphase organ culture method to induce complete mouse spermatogenesis. After incorporating microfluidic systems, we achieved a significant increase in efficiency and duration of spermatogenesis. One of the major drawbacks preventing the popularization of microfluidics, however, is the use of a power-pump to generate medium flow. In this study, we produced a pumpless microfluidic device using hydrostatic pressure and a resistance circuit to facilitate slow, longer lasting medium flow. During three months of culture, results in induction and maintenance of spermatogenesis showed no difference between pumpless and pump-driven devices. Correspondingly, the spermatogonial population was favorably maintained in the pumpless device compared to the conventional method. These results show the advantage of using microfluidic systems for organ culture experiments. Our pumpless device could be applied to a variety of other tissues and organs, and may revolutionize organ culture methods as a whole.
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影响因子：
4.6
作者：
Komeya M;Kimura H;Nakamura H;Yokonishi T;Sato T;Kojima K;Hayashi K;Katagiri K;Yamanaka H;Sanjo H;Yao M;Kamimura S;Inoue K;Ogonuki N;Ogura A;Fujii T;Ogawa T
通讯作者：
Ogawa T
影响因子：
2.6
作者：
Ventel채, S;Mulari, M;Parvinen, M
通讯作者：
Parvinen, M
DOI：
10.1073/pnas.0404353101
发表时间：
2004-11-09
影响因子：
11.1
作者：
Gu, W;Zhu, XY;Takayama, S
通讯作者：
Takayama, S
DOI：
10.1016/j.jala.2007.07.001
发表时间：
2007-12-01
期刊：
JALA (Charlottesville, Va.)
影响因子：
--
作者：
Lee, Philip J;Ghorashian, Navid;Hung, Paul J
通讯作者：
Hung, Paul J
影响因子：
4.2
作者：
Zhu, XY;Chu, LY;Takayama, S
通讯作者：
Takayama, S