Isoflurane modulates neuronal excitability of the nucleus reticularis thalami in vitro.
Isoflurane modulates neuronal excitability of the nucleus reticularis thalami in vitro.
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DOI：
10.1111/j.1749-6632.2009.05172.x
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发表时间：
2010-06
影响因子：
5.2
通讯作者：
Todorovic SM
中科院分区：
文献类型：
--
作者：
Joksovic PM;Todorovic SM
丘脑在处理感觉信息、睡眠和认知方面具有关键功能。我们研究了一种常见的挥发性麻醉剂异氟醚对未成熟大鼠完整脑切片中网状丘脑核（nRT）神经元兴奋性调节的影响。在电流钳记录中，异氟醚（300-600µM）持续去极化膜电位，降低输入电阻，抑制nRT神经元的反弹爆发放电和强直spike放电模式。异氟醚诱导的去极化不仅在河豚毒素存在的情况下持续存在，而且在外源溶液中用Ba2+离子取代Ca2+后也持续存在；细胞外Na+离子部分被n -甲基- d -氨基葡萄糖取代。在电压钳记录中，我们发现异氟醚减缓了t型Ca2+电流失活的恢复。因此，在临床相关浓度下，异氟醚通过多个离子通道抑制发育中的大脑nRT神经元的神经元兴奋性。全身麻醉剂对丘脑细胞神经元兴奋性的抑制可能导致丘脑皮质网络感觉信息传递的损害。这一发现对于理解麻醉的细胞机制，如意识丧失和全身麻醉对哺乳动物大脑发育的潜在破坏性后果可能很重要。
The thalamus has a key function in processing sensory information, sleep, and cognition. We examined the effects of a common volatile anesthetic, isoflurane, on modulation of neuronal excitability in reticular thalamic nucleus (nRT) in intact brain slices from immature rats. In current-clamp recordings, isoflurane (300–600 µM) consistently depolarized membrane potential, decreased input resistance and inhibited both rebound burst firing and tonic spike firing modes of nRT neurons. The isoflurane-induced depolarization persisted not only in the presence of tetrodotoxin, but after replacement of Ca2+ with Ba2+ ions in external solution; it was abolished by partial replacement of extracellular Na+ ions with N-methyl-D-glucamine. In voltage-clamp recordings, we found that isoflurane slowed recovery from inactivation of T-type Ca2+ current. Thus, at clinically relevant concentrations, isoflurane inhibits neuronal excitability of nRT neurons in developing brain via multiple ion channels. Inhibition of the neuronal excitability of thalamic cells may contribute to impairment of sensory information transfer in the thalamocortical network by general anesthetics. The findings may be important for understanding cellular mechanisms of anesthesia such as loss of consciousness and potentially damaging consequences of general anesthetics on developing mammalian brains.
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影响因子：
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