The contribution of sex chromosome conflict to disrupted spermatogenesis in hybrid house mice
The contribution of sex chromosome conflict to disrupted spermatogenesis in hybrid house mice
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性染色体冲突对杂交家鼠精子发生破坏的影响
DOI：
10.1101/2022.07.19.499960
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kopania E
中科院分区：
文献类型：
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作者：
Kopania E
性染色体上的不相容性在杂种雄性不育的进化中很重要，但这种现象背后的进化力量尚不清楚。家鼠（Mus musculus）谱系为理解杂种雄性不育的遗传基础提供了强有力的模型。X染色体-常染色体相互作用导致M. musculusF 1杂种，但不育表型的变化表明一个更复杂的遗传基础。此外，XY染色体的冲突导致了快速扩增的扩增子基因的剂量依赖性表达，这是必不可少的精子发生。在这里，我们评估了XY谱系错配对杂交小鼠雄性生育力和阶段特异性基因表达的贡献。我们在两个家鼠亚种之间进行了回交，以产生相互的Y基因渗入品系，并使用这些品系来测试XY错配在杂交中的影响。我们的转录组分析分选的精子细胞显示广泛的过度表达的X染色体在不育的F1杂种Y染色体亚种的起源无关。因此，减数分裂后的X染色体在不育的F1小鼠杂种的过度表达可能是一个下游的后果破坏减数分裂X失活，而不是XY基因拷贝数失衡。Y染色体渐渗确实导致低生育力表型，并破坏了具有非杂交基因组背景的小鼠中几个常染色体基因的表达，这表明Y连锁不相容性导致生殖障碍，但可能不是XY冲突的直接后果。总的来说，这些研究结果表明，由基因组冲突驱动的性染色体基因家族的快速进化并没有导致这些家鼠亚种之间强烈的雄性生殖障碍。
Incompatibilities on the sex chromosomes are important in the evolution of hybrid male sterility, but the evolutionary forces underlying this phenomenon are unclear. House mice (Mus musculus) lineages have provided powerful models for understanding the genetic basis of hybrid male sterility. X chromosome–autosome interactions cause strong incompatibilities inM. musculusF1 hybrids, but variation in sterility phenotypes suggests a more complex genetic basis. In addition, XY chromosome conflict has resulted in rapid expansions of ampliconic genes with dosage-dependent expression that is essential to spermatogenesis. Here, we evaluated the contribution of XY lineage mismatch to male fertility and stage-specific gene expression in hybrid mice. We performed backcrosses between two house mouse subspecies to generate reciprocal Y-introgression strains and used these strains to test the effects of XY mismatch in hybrids. Our transcriptome analyses of sorted spermatid cells revealed widespread overexpression of the X chromosome in sterile F1 hybrids independent of Y chromosome subspecies origin. Thus, postmeiotic overexpression of the X chromosome in sterile F1 mouse hybrids is likely a downstream consequence of disrupted meiotic X-inactivation rather than XY gene copy number imbalance. Y chromosome introgression did result in subfertility phenotypes and disrupted expression of several autosomal genes in mice with an otherwise nonhybrid genomic background, suggesting that Y-linked incompatibilities contribute to reproductive barriers, but likely not as a direct consequence of XY conflict. Collectively, these findings suggest that rapid sex chromosome gene family evolution driven by genomic conflict has not resulted in strong male reproductive barriers between these subspecies of house mice.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
D. W. Bellott;H. Skaletsky;Ting-Jan Cho;L. Brown;Devin P. Locke;Nancy F. Chen;S. Galkina;Tatyana Pyntikova;Natalia Koutseva;Tina Graves;Colin L. Kremitzki;W. Warren;A. Clark;E. Gaginskaya;R. Wilson;D. Page
通讯作者：
D. W. Bellott;H. Skaletsky;Ting-Jan Cho;L. Brown;Devin P. Locke;Nancy F. Chen;S. Galkina;Tatyana Pyntikova;Natalia Koutseva;Tina Graves;Colin L. Kremitzki;W. Warren;A. Clark;E. Gaginskaya;R. Wilson;D. Page
在自然家鼠杂交区，雄性生育力下降很常见，但其形式和严重程度差异很大
DOI：
--
发表时间：
2012
期刊：
Evolution; international journal of organic evolution
影响因子：
--
作者：
L. Turner;D. Schwahn;B. Harr
通讯作者：
B. Harr
影响因子：
11.8
作者：
Green CD;Ma Q;Manske GL;Shami AN;Zheng X;Marini S;Moritz L;Sultan C;Gurczynski SJ;Moore BB;Tallquist MD;Li JZ;Hammoud SS
通讯作者：
Hammoud SS
影响因子：
3.3
作者：
Rahulsimham Vegesna;Marta Tomaszkiewicz;O. Ryder;Rebeca Campos-Sánchez;P. Medvedev;Michael Degiorgio;K. Makova
通讯作者：
Rahulsimham Vegesna;Marta Tomaszkiewicz;O. Ryder;Rebeca Campos-Sánchez;P. Medvedev;Michael Degiorgio;K. Makova
DOI：
10.1016/j.tig.2018.06.003
发表时间：
2018-09
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Larson EL;Kopania EEK;Good JM
通讯作者：
Good JM