A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.
A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.
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DOI：
10.1016/j.devcel.2018.07.025
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发表时间：
2018-09-10
影响因子：
11.8
通讯作者：
Hammoud SS
中科院分区：
文献类型：
--
作者：
Green CD;Ma Q;Manske GL;Shami AN;Zheng X;Marini S;Moritz L;Sultan C;Gurczynski SJ;Moore BB;Tallquist MD;Li JZ;Hammoud SS
精子发生需要生殖细胞和体细胞之间复杂的相互作用。在生精小管的给定横截面内，多种生殖细胞和体细胞类型共存。这种细胞异质性使得难以在不同的发育阶段描绘不同的细胞类型。为了应对这一挑战，我们从成年小鼠睾丸的约35K细胞中收集了单细胞RNA测序数据，并鉴定了所有已知的生殖细胞和体细胞，以及两种意想不到的体细胞类型。我们的分析揭示了从精原细胞到精子细胞的生殖细胞的连续发育轨迹，并在分化过程中的几个过渡点确定了新的候选转录调控因子。重点分析描绘了四个亚型的精原细胞和九个亚型的支持细胞，后者与组织学定义的发育阶段在生精上皮周期。总的来说，这个高分辨率的细胞图谱代表了研究生殖细胞发育和体内配子发生的社区资源和知识基础。
Spermatogenesis requires intricate interactions between the germline and somatic cells. Within a given cross-section of a seminiferous tubule, multiple germ and somatic cell types co-occur. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. To address this challenge, we collected single-cell RNA sequencing data from ~35K cells from the adult mouse testis, and identified all known germ and somatic cells, as well as two unexpected somatic cell types. Our analysis revealed a continuous developmental trajectory of germ cells from spermatogonia to spermatids, and identified novel candidate transcriptional regulators at several transition points during differentiation. Focused analyses delineated four subtypes of spermatogonia and nine subtypes of Sertoli cells, the latter linked to histologically defined developmental stages over the seminiferous epithelial cycle. Overall, this high-resolution cellular atlas represents a community resource and foundation of knowledge to study germ cell development and in vivo gametogenesis.
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影响因子：
23.9
作者：
Guo J;Grow EJ;Yi C;Mlcochova H;Maher GJ;Lindskog C;Murphy PJ;Wike CL;Carrell DT;Goriely A;Hotaling JM;Cairns BR
通讯作者：
Cairns BR
影响因子：
8.8
作者：
DeFalco T;Potter SJ;Williams AV;Waller B;Kan MJ;Capel B
通讯作者：
Capel B
转录和烙印动力学在发展产后男性系干细胞中。
影响因子：
10.5
作者：
Hammoud SS;Low DH;Yi C;Lee CL;Oatley JM;Payne CJ;Carrell DT;Guccione E;Cairns BR
通讯作者：
Cairns BR
影响因子：
4.5
作者：
Becherel OJ;Yeo AJ;Stellati A;Heng EY;Luff J;Suraweera AM;Woods R;Fleming J;Carrie D;McKinney K;Xu X;Deng C;Lavin MF
通讯作者：
Lavin MF
影响因子：
3.7
作者：
Baskind HA;Na L;Ma Q;Patel MP;Geenen DL;Wang QT
通讯作者：
Wang QT