Analysis of the brain mural cell transcriptome.
Analysis of the brain mural cell transcriptome.
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DOI：
10.1038/srep35108
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发表时间：
2016-10-11
影响因子：
4.6
通讯作者：
Betsholtz C
中科院分区：
文献类型：
--
作者：
He L;Vanlandewijck M;Raschperger E;Andaloussi Mäe M;Jung B;Lebouvier T;Ando K;Hofmann J;Keller A;Betsholtz C
周细胞是微血管的壁细胞，调节微血管的发育和功能，并与许多脑疾病有关。然而，由于缺乏定义标记，周细胞的识别和功能表征仍然模糊和数据解释的问题。在携带两种转基因报告基因Pdgfrb-eGFP和NG 2-DsRed的小鼠中，我们发现双阳性细胞是血管壁细胞，而单报告基因标记了额外的但不重叠的神经胶质细胞。通过荧光激活细胞分选（FACS）分离双阳性细胞并通过RNA测序分析。为了揭示壁细胞转录物的定义模式，我们将RNA测序数据与先前发表的四项研究的数据进行了比较。荟萃分析提供了260个脑壁细胞富集基因转录本的保守目录。我们验证了周细胞特异性表达的两个新的标志物，玻连蛋白（Vtn）和干扰素诱导的跨膜蛋白1（IFITM 1），使用荧光原位杂交和免疫组织化学。我们进一步分析了周细胞富集基因的信号通路和相互作用网络。这项工作提供了新的见解脑壁细胞的分子组成。报道的基因目录通过提供许多新的候选标记基因促进了脑周细胞的鉴定，并且为未来脑壁细胞生理学和病理生理学的研究提供了新假设的丰富来源。
Pericytes, the mural cells of blood microvessels, regulate microvascular development and function and have been implicated in many brain diseases. However, due to a paucity of defining markers, pericyte identification and functional characterization remain ambiguous and data interpretation problematic. In mice carrying two transgenic reporters, Pdgfrb-eGFP and NG2-DsRed, we found that double-positive cells were vascular mural cells, while the single reporters marked additional, but non-overlapping, neuroglial cells. Double-positive cells were isolated by fluorescence-activated cell sorting (FACS) and analyzed by RNA sequencing. To reveal defining patterns of mural cell transcripts, we compared the RNA sequencing data with data from four previously published studies. The meta-analysis provided a conservative catalogue of 260 brain mural cell-enriched gene transcripts. We validated pericyte-specific expression of two novel markers, vitronectin (Vtn) and interferon-induced transmembrane protein 1 (Ifitm1), using fluorescent in situ hybridization and immunohistochemistry. We further analyzed signaling pathways and interaction networks of the pericyte-enriched genes in silico. This work provides novel insight into the molecular composition of brain mural cells. The reported gene catalogue facilitates identification of brain pericytes by providing numerous new candidate marker genes and is a rich source for new hypotheses for future studies of brain mural cell physiology and pathophysiology.
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影响因子：
4.8
作者：
Bondjers, Cecilia;He, Liqun;Betsholtz, Christer
通讯作者：
Betsholtz, Christer
影响因子：
16.6
作者：
Follmann, Markus;Griebenow, Nils;Straub, Alexander
通讯作者：
Straub, Alexander
DOI：
10.1023/a:1011965307612
发表时间：
2001-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Bandopadhyay, R;Orte, C;Allt, G
通讯作者：
Allt, G
影响因子：
1.9
作者：
Dore-Duffy, Paula;Wang, Xueqain;Rafols, Jose A.
通讯作者：
Rafols, Jose A.
影响因子：
3.7
作者：
Daneman R;Zhou L;Agalliu D;Cahoy JD;Kaushal A;Barres BA
通讯作者：
Barres BA