Vaccinia virus strain differences in cell attachment and entry.
Vaccinia virus strain differences in cell attachment and entry.
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DOI：
10.1016/j.virol.2009.04.012
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发表时间：
2009-06-20
期刊：
影响因子：
3.7
通讯作者：
Moss, Bernard
中科院分区：
文献类型：
--
作者：
Bengali, Zain;Townsley, Alan C.;Moss, Bernard
痘苗病毒(VACV)WR株可通过低pH内体途径进入细胞，也可在中性pH条件下直接与质膜融合。在这里，我们比较了五个VACV毒株在六个细胞系中的附着和进入，发现了两种主要的模式。只有WR在中性pH吸附到细胞后表现出pH 5增强的进入速率，这与内体酸化抑制剂巴菲罗霉素A1的敏感性有关。PH 5处理既不能加速IHD-J、哥本哈根和Elstree菌株的进入，也不能阻止巴菲霉素A1的进入。惠氏菌株的进入，虽然没有被pH 5增强，但被巴菲罗星A1抑制。WR和Wyeth对肝素进入的负面影响都相对耐受，而其他菌株由于抑制细胞结合而极其敏感。单个痘苗病毒株对肝素的相对敏感性与它们与糖胺多糖缺陷的sog9细胞结合和进入的能力成反比，但与其他被测试的细胞系无关。这些结果表明，与WR和Wyeth相比，IHD-J、哥本哈根和Elstree利用低pH内体途径的能力更有限，并且更依赖于与糖胺多聚糖结合来实现细胞附着。
Vaccinia virus (VACV) strain WR can enter cells by a low pH endosomal pathway or direct fusion with the plasma membrane at neutral pH. Here, we compared attachment and entry of five VACV strains in six cell lines and discovered two major patterns. Only WR exhibited pH 5-enhanced rate of entry following neutral pH adsorption to cells, which correlated with sensitivity to bafilomycin A1, an inhibitor of endosomal acidification. Entry of IHD-J, Copenhagen and Elstree strains were neither accelerated by pH 5 treatment nor prevented by bafilomycin A1. Entry of the Wyeth strain, although not augmented by pH 5, was inhibited by bafilomycin A1. WR and Wyeth were both relatively resistant to the negative effects of heparin on entry, whereas the other strains were extremely sensitive due to inhibition of cell binding. The relative sensitivities of individual vaccinia virus strains to heparin correlated inversely with their abilities to bind to and enter glycosaminoglycan-deficient sog9 cells but not other cell lines tested. These results suggested that that IHD-J, Copenhagen and Elstree have a more limited ability than WR and Wyeth to use the low pH endosomal pathway and are more dependent on binding to glycosaminoglycans for cell attachment.
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