New techniques for creating parthenogenetic larvae of the sea urchin Lytechinus pictus for gene expression studies.
New techniques for creating parthenogenetic larvae of the sea urchin Lytechinus pictus for gene expression studies.
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DOI:
10.1002/dvdy.377
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发表时间:
2021-12
期刊:
影响因子:
--
通讯作者:
Hamdoun A
中科院分区:
文献类型:
--
作者:
Vacquier VD;Hamdoun A
Sea urchins are model organisms for studying the spatial-temporal control of gene activity during development. The Southern California species, Lytechinus pictus, has a sequenced genome and can be raised in the laboratory from egg to egg in 4–5 months. Here, we present new techniques for generating parthenogenetic larvae of this species and include a gallery of photomicrographs of morphologically abnormal larvae that could be used for transcriptomic analysis. Comparison of gene expression in parthenogenotes to larvae produced by fertilization could provide novel insights into gene expression controls contributed by sperm in this important model organism. Knowledge gained from transcriptomics of sea urchin parthenogenotes could contribute to parthenogenetic studies of mammalian embryos.
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影响因子:
4.6
作者:
Foster, Stephany;Oulhen, Nathalie;Wessel, Gary
通讯作者:
Wessel, Gary
影响因子:
1.5
作者:
CAMERON, RA;HINEGARDNER, RT
通讯作者:
HINEGARDNER, RT
影响因子:
3.3
作者:
Warner, Jacob F.;Lord, James W.;Lyons, Deirdre C.
通讯作者:
Lyons, Deirdre C.
DOI:
10.1007/978-1-0716-0779-4_23
发表时间:
2021
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
作者:
Massri AJ;Schiebinger GR;Berrio A;Wang L;Wray GA;McClay DR
通讯作者:
McClay DR
影响因子:
1.4
作者:
BURKE, RD
通讯作者:
BURKE, RD