Concurrent measurement of dynamic changes in viral load, serum enzymes, T cell subsets, and cytokines in patients with severe fever with thrombocytopenia syndrome.
Concurrent measurement of dynamic changes in viral load, serum enzymes, T cell subsets, and cytokines in patients with severe fever with thrombocytopenia syndrome.
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DOI：
10.1371/journal.pone.0091679
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Huang Z
中科院分区：
文献类型：
--
作者：
Li J;Han Y;Xing Y;Li S;Kong L;Zhang Y;Zhang L;Liu N;Wang Q;Wang S;Lu S;Huang Z
严重发热伴血小板减少综合征（SFTS）是一种新型布尼亚病毒引起的新发感染。在同期研究中，缺乏对与SFTS相关的临床、实验室和免疫学异常的动态变化的分析。33例SFTS患者在江苏省南京市人民医院住院治疗，根据临床症状和RT-PCR检测病毒RNA阳性作出诊断。4名患者死亡，29人存活。从发烧开始的第5天至第15天期间每隔一天采集一次血液样本。健康志愿者的样本用作正常对照。死亡患者的病毒RNA载量峰值、血清酶、IL-6和IL-10显著高于存活者。病毒载量，血清酶和细胞因子下降的幸存者在2周内从发烧开始。CD 69 + T细胞在感染后早期升高，而HLA-DR+和CTLA 4 + T细胞在存活者的恢复期升高。同时观察到高水平的SFTSV病毒载量、PLT降低、血清酶升高、促炎和抗炎细胞因子升高以及CD 69 + T细胞活化。这些参数的变化程度和模式可能指示SFTSV感染患者的临床结局。
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infection caused by a novel Bunyavirus. Analysis on the dynamic changes of clinical, laboratory, and immunological abnormalities associated with SFTS in a concurrent study is lacking. Thirty-three SFTS patients were admitted to Jiangsu People's Hospital, Nanjing, China, and diagnosis was made based on the clinical symptoms and positive viral RNA detected by RT-PCR. Four patients deceased and twenty-nine survived. Blood samples were collected every other day between Day 5 and Day 15 from the onset of fever. Samples from healthy volunteers were used as normal controls. Peak viral RNA load, serum enzymes, IL-6, and IL-10 were significantly higher in deceased patients compared to survivors. Viral load, serum enzymes, and cytokines declined in survivors within 2 weeks from onset of fever. CD69+ T cells were elevated early after infection while HLA-DR+ and CTLA4+ T cells were elevated during the recovery phase of those who survived. High level SFTSV viral load was concurrently observed with reduced PLT, elevated serum enzymes, elevated pro-inflammatory and anti-inflammatory cytokines, and activation of CD69+ T cells. The degree and pattern of changes in these parameters may indicate the clinical outcome in SFTSV-infected patients.
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影响因子：
11.8
作者：
Zhang, Yong-Zhen;He, Yong-Wen;Xu, Jianguo
通讯作者：
Xu, Jianguo
影响因子：
3.7
作者：
Deng B;Zhou B;Zhang S;Zhu Y;Han L;Geng Y;Jin Z;Liu H;Wang D;Zhao Y;Wen Y;Cui W;Zhou Y;Gu Q;Sun C;Lu X;Wang W;Wang Y;Li C;Wang Y;Yao W;Liu P
通讯作者：
Liu P
中国一种新型布尼亚病毒引起的发热伴血小板减少症。
DOI：
10.1056/nejmoa1010095
发表时间：
2011-04-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Yu XJ;Liang MF;Zhang SY;Liu Y;Li JD;Sun YL;Zhang L;Zhang QF;Popov VL;Li C;Qu J;Li Q;Zhang YP;Hai R;Wu W;Wang Q;Zhan FX;Wang XJ;Kan B;Wang SW;Wan KL;Jing HQ;Lu JX;Yin WW;Zhou H;Guan XH;Liu JF;Bi ZQ;Liu GH;Ren J;Wang H;Zhao Z;Song JD;He JR;Wan T;Zhang JS;Fu XP;Sun LN;Dong XP;Feng ZJ;Yang WZ;Hong T;Zhang Y;Walker DH;Wang Y;Li DX
通讯作者：
Li DX
影响因子：
11.8
作者：
Bao, Chang-jun;Guo, Xi-ling;Wang, Hua
通讯作者：
Wang, Hua
影响因子：
12.7
作者：
Huang, KJ;Su, IJ;Lei, HY
通讯作者：
Lei, HY