Laboratory Diagnosis and Monitoring the Viral Shedding of SARS-CoV-2 Infection.
Laboratory Diagnosis and Monitoring the Viral Shedding of SARS-CoV-2 Infection.
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实验室诊断和监测SARS-COV-2感染的病毒脱落。
DOI：
10.1016/j.xinn.2020.100061
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发表时间：
2020-11-25
期刊：
影响因子：
--
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Yang Y;Yang M;Yuan J;Wang F;Wang Z;Li J;Zhang M;Xing L;Wei J;Peng L;Wong G;Zheng H;Wu W;Shen C;Liao M;Feng K;Li J;Yang Q;Zhao J;Liu L;Liu Y
2019冠状病毒病（COVID-19）的全球流行仍在持续。快速准确地检测SARS-CoV-2致病病毒对于治疗和控制COVID-19至关重要。本研究使用广东省疾病预防控制中心确诊的410例COVID-19患者的3，552份临床样本，回顾性分析了不同呼吸道标本类型的比较敏感性。发病后14天内，除支气管肺泡灌洗液（BALF）外，痰标本的阳性率最高（73.4%~ 87.5%），其次为鼻拭子（53.1%~ 85.3%）。在14 d.a.o.内从严重组收集的所有BALF样品中可以检测到病毒RNA。持续了46天此外，虽然病毒RNA在上呼吸道样本中为阴性，但在治疗期间，重度组的大多数病例的BALF样本中也为阳性。值得注意的是，在轻度组的BALF样品中未检测到病毒RNA。尽管通过计算机断层扫描观察到典型的毛玻璃样混浊，但在一些COVID-19患者的前三个或所有上呼吸道标本中均未检测到病毒RNA。总之，痰液对COVID-19的常规实验室诊断最敏感，其次是鼻拭子。BALF中病毒RNA的检测提高了重症COVID-19患者的诊断准确性。痰液中SARS-CoV-2 RNA的检测对COVID-19的常规实验室诊断最为敏感，鼻咽拭子中病毒的脱落情况在轻、重症病例之间有显著差异。BALF中病毒RNA的检测（支气管肺泡灌洗液）提高了严重病例的诊断准确性计算机断层扫描（CT）扫描可作为分子诊断的补充工具
The worldwide epidemic of coronavirus disease 2019 (COVID-19) is ongoing. Rapid and accurate detection of the causative virus SARS-CoV-2 is vital for the treatment and control of COVID-19. In this study, the comparative sensitivity of different respiratory specimen types were retrospectively analyzed using 3,552 clinical samples from 410 COVID-19 patients confirmed by Guangdong CDC (Center for Disease Control and Prevention). Except for bronchoalveolar lavage fluid (BALF), the sputum possessed the highest positive rate (73.4%–87.5%), followed by nasal swabs (53.1%–85.3%) for both severe and mild cases during the first 14 days after illness onset (d.a.o.). Viral RNA could be detected in all BALF samples collected from the severe group within 14 d.a.o. and lasted up to 46 d.a.o. Moreover, although viral RNA was negative in the upper respiratory samples, it was also positive in BALF samples in most cases from the severe group during treatment. Notably, no viral RNA was detected in BALF samples from the mild group. Despite typical ground-glass opacity observed via computed tomographic scans, no viral RNA was detected in the first three or all upper respiratory tract specimens from some COVID-19 patients. In conclusion, sputum is most sensitive for routine laboratory diagnosis of COVID-19, followed by nasal swabs. Detection of viral RNA in BALF improves diagnostic accuracy in severe COVID-19 patients. Detecting SARS-CoV-2 RNA in sputum was most sensitive for routine laboratory diagnosis of COVID-19, followed by nasopharyngeal swabs Viral shedding profiles of the upper and lower respiratory tract were significantly different between severe and mild cases Detection of viral RNA in BALF (bronchoalveolar lavage fluid) improves diagnostic accuracy in severe cases Computed tomography (CT) scan may serve as a complementary tool for the molecular diagnosis
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影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
DOI：
10.1007/s10096-005-1299-5
发表时间：
2005-03
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Xu D;Zhang Z;Jin L;Chu F;Mao Y;Wang H;Liu M;Wang M;Zhang L;Gao GF;Wang FS
通讯作者：
Wang FS
影响因子：
82.9
作者：
He, Xi;Lau, Eric H. Y.;Leung, Gabriel M.
通讯作者：
Leung, Gabriel M.
人感染H5N6禽流感病毒的临床和免疫学特征
影响因子：
11.8
作者：
Bi, Yuhai;Tan, Shuguang;Liu, Yingxia
通讯作者：
Liu, Yingxia
影响因子：
12.7
作者：
Zhang, JingCheng;Wang, SaiBin;Xue, YaDong
通讯作者：
Xue, YaDong