Establishment of a Polymerase Chain Reaction Method for Detection of Escherichia coli in Amniotic Fluid in Patients with Chorioamnionitis
Establishment of a Polymerase Chain Reaction Method for Detection of Escherichia coli in Amniotic Fluid in Patients with Chorioamnionitis
复制标题
绒毛膜羊膜炎患者羊水中大肠杆菌聚合酶链反应检测方法的建立
DOI：
10.1159/000142143
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发表时间：
2008
影响因子：
2.2
通讯作者：
M. Tanemura
中科院分区：
文献类型：
--
作者：
G. A. Daoud;Yoshikatsu Suzuki;Tamao Yamamoto;Takashi Suzuki;N. Suzumori;M. Tanemura
目的：探讨大肠埃希菌能否被检出。方法应用聚合酶链反应（PCR）检测羊水中大肠杆菌的大肠杆菌参考群（ECOR）和毒力因子（VF）。方法：从18例临床症状，如周期性子宫收缩，生殖器出血和宫颈成熟，随后发生流产或分娩前，和40例正常孕妇进行诊断性羊水穿刺，羊水。所有样本均为标准培养阴性。ECOR组6例，其中VF组、E.大肠埃希菌阳性6例。因此，4例患者两项试验均呈阳性。结论：建立了大肠埃希菌的检测方法。使用ECOR分组和PCR检测羊水中的大肠杆菌。
Objective: We investigated whether Escherichia coli could be detected by E. coli of reference (ECOR) grouping and virulence factors (VFs) in amniotic fluid using polymerase chain reaction (PCR). Method: From 18 patients with clinical symptoms, such as cyclic uterine contraction, genital bleeding and cervical ripening, who subsequently developed abortion or labor before term, and from 40 normal pregnant women undergoing diagnostic amniocentesis, amniotic fluid was obtained. All samples were negative for standard culture. Six patients with symptoms were classified into the ECOR group, and with VFs, E. coli was detected in 6 patients. Thus, 4 patients were positive for both tests. Conclusion: We could establish a detection method for E. coli in amniotic fluid using both ECOR grouping and VFs with PCR.
影响因子：
6.4
作者：
Johnson, JR;Stell, AL
通讯作者：
Stell, AL
DOI：
10.1016/0002-9378(93)90014-a
发表时间：
1993-10-01
影响因子：
9.8
作者：
ROMERO, R;YOON, BH;SEHGAL, P
通讯作者：
SEHGAL, P