The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen.
The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen.
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DOI：
10.1093/nar/gki297
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发表时间：
2005
影响因子：
14.9
通讯作者：
Lee YS
中科院分区：
文献类型：
--
作者：
Chiu CH;Tang P;Chu C;Hu S;Bao Q;Yu J;Chou YY;Wang HS;Lee YS
猪霍乱沙门氏菌（Salmonella enterica serovar Choleraesuis，S.Choleraesuis）是非伤寒沙门氏菌中一种高度侵袭性的血清型，通常会导致人类败血症或肠外病灶感染。猪霍乱沙门氏菌感染现在变得特别难以治疗，因为出现了对多种抗菌药物的耐药性。测定了多重耐药猪霍乱沙门氏菌SC-B67株的4.7Mb基因组序列。三种测序沙门氏菌基因组的全基因组比较显示，相对于鼠伤寒沙门氏菌LT 2，猪霍乱沙门氏菌SC-B67和伤寒沙门氏菌CT 18发生更多的缺失事件。猪霍乱沙门氏菌有151个假基因，其中，在三个沙门氏菌基因组中，包括最高比例的假基因所产生的基因参与细菌趋化性信号转导途径。这些基因的突变可能会增加细菌的平滑游动，从而可能使其与宿主细胞发生更有效的相互作用并入侵宿主细胞。TetR/AcrR家族的关键调控基因acrR通过引入内部终止密码子而失活，导致AcrAB过表达，这似乎与环丙沙星抗性相关。虽然在沙门氏菌的不同血清型的进化过程中维持了提供基本功能以允许宿主和环境中的生态位扩展的横向基因转移，但提供很少总体选择性益处的基因可能会迅速丧失。我们的研究结果表明，假基因的形成可能提供了一个简单的进化途径，补充基因的收购，以提高毒力和耐药性的猪霍乱弧菌。
Salmonella enterica serovar Choleraesuis (S.Choleraesuis), a highly invasive serovar among non-typhoidal Salmonella, usually causes sepsis or extra-intestinal focal infections in humans. S.Choleraesuis infections have now become particularly difficult to treat because of the emergence of resistance to multiple antimicrobial agents. The 4.7 Mb genome sequence of a multidrug-resistant S.Choleraesuis strain SC-B67 was determined. Genome wide comparison of three sequenced Salmonella genomes revealed that more deletion events occurred in S.Choleraesuis SC-B67 and S.Typhi CT18 relative to S.Typhimurium LT2. S.Choleraesuis has 151 pseudogenes, which, among the three Salmonella genomes, include the highest percentage of pseudogenes arising from the genes involved in bacterial chemotaxis signal-transduction pathways. Mutations in these genes may increase smooth swimming of the bacteria, potentially allowing more effective interactions with and invasion of host cells to occur. A key regulatory gene of TetR/AcrR family, acrR, was inactivated through the introduction of an internal stop codon resulting in overexpression of AcrAB that appears to be associated with ciprofloxacin resistance. While lateral gene transfer providing basic functions to allow niche expansion in the host and environment is maintained during the evolution of different serovars of Salmonella, genes providing little overall selective benefit may be lost rapidly. Our findings suggest that the formation of pseudogenes may provide a simple evolutionary pathway that complements gene acquisition to enhance virulence and antimicrobial resistance in S.Choleraesuis.
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影响因子：
3.1
作者：
Haneda, T;Okada, N;Danbara, H
通讯作者：
Danbara, H
DOI：
10.1073/pnas.93.19.10303
发表时间：
1996-09-17
影响因子：
11.1
作者：
Liu, SL;Sanderson, KE
通讯作者：
Sanderson, KE
影响因子：
3.8
作者：
KHORAMIANFALSAFI, T;HARAYAMA, S;PECHERE, JC
通讯作者：
PECHERE, JC
影响因子：
4.9
作者：
Barnaud, G;Arlet, G;Philippon, A
通讯作者：
Philippon, A
影响因子：
3.1
作者：
Chu, CS;Hong, SF;Ou, JT
通讯作者：
Ou, JT