Self-repair protects microtubules from destruction by molecular motors.
Self-repair protects microtubules from destruction by molecular motors.
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DOI：
10.1038/s41563-020-00905-0
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发表时间：
2021-06
期刊：
影响因子：
41.2
通讯作者：
Théry M
中科院分区：
文献类型：
--
作者：
Triclin S;Inoue D;Gaillard J;Htet ZM;DeSantis ME;Portran D;Derivery E;Aumeier C;Schaedel L;John K;Leterrier C;Reck-Peterson SL;Blanchoin L;Théry M
微管的不稳定性源于微管蛋白二聚体相互作用的低能量，这使得生长中的聚合物接近其分解条件。分子马达利用ATP水解产生机械功并在微管上运动。这增加了行走马达产生的机械功可能会破坏二聚体相互作用并引发微管拆卸的可能性。我们通过在体外研究微管和运动分子马达之间的相互作用来验证这一假设。我们的研究结果表明，分子马达可以从晶格中去除微管蛋白二聚体，并迅速破坏微管。我们还发现马达去除二聚体补偿了微管晶格中游离微管蛋白二聚体的插入。这种自我修复机制使微管能够在分子马达沿着其轨迹运动时所引起的损伤中存活下来。我们的研究揭示了分子马达的运动与微管晶格的更新之间存在耦合。
Microtubule instability stems from the low energy of tubulin dimer interactions, which sets the growing polymer close to its disassembly conditions. Molecular motors use ATP hydrolysis to produce mechanical work and move on microtubules. This raises the possibility that the mechanical work produced by walking motors can break dimer interactions and trigger microtubule disassembly. We tested this hypothesis by studying the interplay between microtubules and moving molecular motors in vitro. Our results show that molecular motors can remove tubulin dimers from the lattice and rapidly destroy microtubules. We also found that dimer removal by motors was compensated for the insertion of free tubulin dimers into the microtubule lattice. This self-repair mechanism allows microtubules to survive the damage induced by molecular motors as they move along their tracks. Our study reveals the existence of coupling between the motion of molecular motors and the renewal of the microtubule lattice.
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DOI：
10.1083/jcb.201104034
发表时间：
2011-07-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nakata T;Niwa S;Okada Y;Perez F;Hirokawa N
通讯作者：
Hirokawa N
影响因子：
7.7
作者：
Hibbel, Anneke;Bogdanova, Aliona;Howard, Jonathan
通讯作者：
Howard, Jonathan
影响因子：
16
作者：
Hunter, AW;Caplow, M;Howard, J
通讯作者：
Howard, J
影响因子：
56.9
作者：
Carazo-Salas, RE;Antony, C;Nurse, P
通讯作者：
Nurse, P
影响因子：
9.2
作者：
de Forges, Helene;Pilon, Antoine;Pous, Christian
通讯作者：
Pous, Christian