Alpha-Glucan, Water Dikinase 1 Affects Starch Metabolism and Storage Root Growth in Cassava (Manihot esculenta Crantz).
Alpha-Glucan, Water Dikinase 1 Affects Starch Metabolism and Storage Root Growth in Cassava (Manihot esculenta Crantz).
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α-葡聚糖、水二激酶 1 影响木薯 (Manihot esculenta Crantz) 的淀粉代谢和贮藏根生长
DOI：
10.1038/s41598-017-10594-6
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发表时间：
2017-08-29
影响因子：
4.6
通讯作者：
Zhang P
中科院分区：
文献类型：
--
作者：
Zhou W;He S;Naconsie M;Ma Q;Zeeman SC;Gruissem W;Zhang P
源强对贮藏根发育的调节作用在很大程度上还不清楚。木薯贮藏根延迟（srd）T-DNA突变体推迟贮藏根的发展，但表现出正常的叶片生长的野生型植物。SRD基因被鉴定为α-葡聚糖的直向同源物，水双激酶1（GWD 1），其表达在叶片中的光/暗循环条件下被调节，并且与贮藏根的发育相关。GWD 1-RNAi木薯植物表现出植物和贮藏根生长迟缓，这是淀粉过量表型的结果，其光合能力降低，叶片中可溶性淀粉水平降低。这些叶子含有淀粉颗粒，具有大大增加的直链淀粉含量和具有增加的短链的C型半结晶结构，这表明储存淀粉。在GWD 1-RNAi株系的贮藏根中，麦芽糖含量显著降低，磷酸化水平低得多的淀粉显示出显著降低的β-淀粉分解速率。这些结果表明，GWD 1调节瞬时淀粉形态建成和储存根的生长，减少从源到库的光同化分配和淀粉动员在块根作物。
AbstarctRegulation of storage root development by source strength remains largely unknown. The cassava storage root delay (srd) T-DNA mutant postpones storage root development but manifests normal foliage growth as wild-type plants. The SRD gene was identified as an orthologue of α-glucan, water dikinase 1 (GWD1), whose expression is regulated under conditions of light/dark cycles in leaves and is associated with storage root development. The GWD1-RNAi cassava plants showed both retarded plant and storage root growth, as a result of starch excess phenotypes with reduced photosynthetic capacity and decreased levels of soluble saccharides in their leaves. These leaves contained starch granules having greatly increased amylose content and type C semi-crystalline structures with increased short chains that suggested storage starch. In storage roots of GWD1-RNAi lines, maltose content was dramatically decreased and starches with much lower phosphorylation levels showed a drastically reduced β-amylolytic rate. These results suggested that GWD1 regulates transient starch morphogenesis and storage root growth by decreasing photo-assimilation partitioning from the source to the sink and by starch mobilization in root crops.
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影响因子：
7.4
作者：
Kötting, O;Pusch, K;Ritte, G
通讯作者：
Ritte, G
影响因子：
4.4
作者：
An D;Yang J;Zhang P
通讯作者：
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影响因子：
7.4
作者：
Edner, Christoph;Li, Jing;Ritte, Gerhard
通讯作者：
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影响因子：
46.9
作者：
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通讯作者：
Kossmann, J
影响因子：
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作者：
Mikkelsen, R;Blennow, A
通讯作者：
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