cDNA cloning and tissue-specific regulation of expression of rat calcium-binding protein 65/67. Identification as a homologue of annexin VI.
cDNA cloning and tissue-specific regulation of expression of rat calcium-binding protein 65/67. Identification as a homologue of annexin VI.
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大鼠钙结合蛋白 65/67 的 cDNA 克隆和表达的组织特异性调节。
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
W. Reutter
中科院分区：
文献类型：
--
作者：
H. Fan;D. Josić;Y. Lim;W. Reutter
利用单特异性多克隆抗（CBP 65/67）抗体和单克隆抗（CBP 65/67） IgG免疫筛选，从大鼠肝脏lambda ZAP II cDNA表达文库中分离出大鼠膜相关65/67 kda钙结合蛋白CBP 65/67。该cDNA在大肠杆菌中表达的产物经免疫染色和SDS/PAGE与从大鼠肝脏中分离的CBP 65/67分子质量比较证实为CBP 65/67。CBP 65/67的cDNA序列和推导出的氨基酸序列均与人类p68和钙电蛋白具有高度的同源性，这两种蛋白属于钙依赖、膜相关、磷脂结合蛋白家族，称为膜联蛋白。这意味着CBP 65/67是上述两种人类蛋白的同源物。我们不知道大鼠膜联蛋白VI先前已被分离和测序。采用Northern blot方法研究了CBP 65/67 mRNA在大鼠不同器官发育过程中的表达情况。在成人组织中，肺、心脏、肌肉、脾脏，尤其是胸腺和胰腺，CBP 65/67 mRNA水平较高，而在肝、肾、肠、胃和脑中，CBP 65/67 mRNA水平较低。肾脏、胃和肌肉组织发育过程中mRNA的表达量变化不大。相比之下，CBP 65/67在肝、肺、心和脑中的表达明显增加。在研究的大多数器官中，mRNA水平与蛋白表达水平密切相关，表明CBP 65/67在大多数器官中的表达主要受转录水平的控制。
We isolated a cDNA encoding the rat membrane-associated 65/67-kDa calcium-binding protein, CBP 65/67, from a lambda ZAP II cDNA-expression library of rat liver by immunoscreening using monospecific polyclonal anti-(CBP 65/67) antibodies and monoclonal anti-(CBP 65/67) IgG. The product of this cDNA expressed in Escherichia coli was confirmed as CBP 65/67 both by immunostaining and by comparison of the molecular mass with the CBP 65/67 isolated from rat liver by SDS/PAGE. The cDNA sequence and the deduced amino acid sequence of CBP 65/67 both show a high degree of identity to human p68 and human calelectrin, which belong to a family of calcium-dependent, membrane-associated, phospholipid-binding proteins, called annexins. This means that CBP 65/67 is a homolog of the two human proteins just mentioned above. We are not aware that a rat annexin VI has previously been isolated and sequenced. The mRNA expression of CBP 65/67 in different rat organs during development was investigated by Northern blot analysis. In adult tissues, high mRNA levels of CBP 65/67 were found in lung, heart, muscle, spleen and especially in thymus and pancreas, whereas in liver, kidney, intestine, stomach and brain only low levels of CBP 65/67 mRNA could be detected. The amount of mRNA during tissue development in kidney, stomach and muscle showed only slight changes. In contrast, a significant increase of CBP 65/67 expression was observed in liver, lung, heart and brain. In most of the organs investigated, the level of mRNA correlated closely with the level of protein expression, indicating that the expression of CBP 65/67 in most organs is controlled primarily at the transcriptional level.
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通过二维等电聚焦/十二烷基硫酸钠凝胶电泳分离后根据生物活性鉴定蛋白质：人外分泌胰腺蛋白的分析。
影响因子：
2.9
作者：
Scheele,G;Pash,J;Bieger,W
通讯作者：
Bieger,W
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
DOI：
10.1016/s0021-9258(19)38604-1
发表时间：
1990-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Johnston;M. Perin;G. A. Reynolds;S. Wasserman;T. Südhof
通讯作者：
P. Johnston;M. Perin;G. A. Reynolds;S. Wasserman;T. Südhof
DOI：
10.1016/s0021-9258(18)38177-8
发表时间：
1990-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Perumal Thiagarajan;Jonathan F. Tait
通讯作者：
Perumal Thiagarajan;Jonathan F. Tait
Ca(2 ) 依赖性膜联蛋白在膜表面的自缔合。
影响因子：
2.9
作者：
Zaks,WJ;Creutz,CE
通讯作者：
Creutz,CE