DNA lesions in human neoplastic cells and cytotoxicity of 5-fluoropyrimidines.
DNA lesions in human neoplastic cells and cytotoxicity of 5-fluoropyrimidines.
复制标题
人类肿瘤细胞中的 DNA 损伤和 5-氟嘧啶的细胞毒性。
作者：
U. Lönn;S. Lönn
我们检查了用 5-氟尿嘧啶和 5-氟脱氧尿苷处理的人类肿瘤细胞 DNA 中碱不稳定区域的诱导情况。 5-氟尿嘧啶通过两种机制诱导DNA损伤：将药物掺入DNA中，以及第二种不涉及掺入的机制。第二种机制是在用 aphidicolin（一种 DNA 聚合酶 α 的特异性抑制剂）处理的细胞中观察到的，可阻止 DNA 复制叉的运动。 5-氟脱氧尿苷没有掺入这些细胞的 DNA 中；仅检测到碱不稳定DNA的第二种诱导机制。第二种机制很可能是由于嘌呤和嘧啶残基中正常发生的缺陷的 DNA 修复效率低下。此外，DNA 中碱不稳定区域水平的增加与药物的细胞毒性之间存在相关性。这可能是 5-氟嘧啶的杀细胞作用的一种解释。
We have examined the induction of alkali-labile regions in DNA of human neoplastic cells treated with 5-fluorouracil and 5-fluorodeoxyuridine. 5-Fluorouracil induces DNA lesions by two mechanisms: incorporation of drug into DNA and a second mechanism not involving the incorporation. The second mechanism is seen in cells treated with aphidicolin, a specific inhibitor of DNA polymerase alpha, to stop the movement of the DNA replication forks. 5-Fluorodeoxyuridine is not incorporated into DNA of these cells; only the second mechanism of induction of alkali-labile DNA is detected. The second mechanism is in all probability due to inefficient DNA repair of normally occurring defects in purine and pyrimidine residues. Furthermore there is a correlation between increasing levels of alkali-labile regions in the DNA and cytotoxicity of the drugs. This may be one explanation for the cytocidal effects of 5-fluoropyrimidines.
影响因子：
11.2
作者：
P. Major;E. Egan;D. Herrick;D. Kufe
通讯作者：
P. Major;E. Egan;D. Herrick;D. Kufe
5-氟-2-脱氧尿苷对 HeLa 细胞 DNA 代谢的影响。
影响因子：
3.6
作者：
Cheng,YC;Nakayama,K
通讯作者：
Nakayama,K
影响因子：
14.9
作者：
Pettersen HS;Visnes T;Vågbø CB;Svaasand EK;Doseth B;Slupphaug G;Kavli B;Krokan HE
通讯作者：
Krokan HE