Abolished ketamine effects on the spontaneous excitatory postsynaptic current of medial prefrontal cortex neurons in GluN2D knockout mice.
Abolished ketamine effects on the spontaneous excitatory postsynaptic current of medial prefrontal cortex neurons in GluN2D knockout mice.
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DOI：
10.1186/s13041-021-00883-7
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发表时间：
2021-12-07
期刊：
影响因子：
3.6
通讯作者：
Kaang BK
中科院分区：
文献类型：
--
作者：
Han DH;Hong I;Choi JE;Park P;Baek JY;Park H;Ide S;Mishina M;Ikeda K;Kaang BK
氯胺酮是N-甲基-d-天冬氨酸受体(NMDAR)的非竞争性拮抗剂，可产生快速有效的抗抑郁作用。它发挥了心理拟态效应，但其作用机制还有待进一步研究。先前的研究表明，氯胺酮不再促进GluN2D基因敲除(KO)小鼠的多动，这是NMDAR的一个亚单位。在本研究中，我们测试了含有GluN2D的NMDAR是否参与了氯胺酮引发的内侧前额叶皮质(MPFC)的生理变化。亚麻剂量氯胺酮(25 mg/kg)可使野生型(WT)小鼠的自发兴奋性突触后电流(SEPSC)频率升高，但对GluN2D KO小鼠无明显影响。氯胺酮不影响WT和GluN2D KO小鼠的sEPSC波幅和配对脉搏比(PPR)。这些发现表明，含有GluN2D的NMDAR可能在氯胺酮引起的mPFC谷氨酸能神经元的改变中起作用，并可能在氯胺酮引起的多动反应中起作用。
Ketamine, a non-competitive antagonist of the N-methyl-d-aspartate receptor (NMDAR), generates a rapidly-acting antidepressant effect. It exerts psychomimetic effects, yet demands a further investigation of its mechanism. Previous research showed that ketamine did no longer promote hyperlocomotion in GluN2D knockout (KO) mice, which is a subunit of NMDAR. In the present study, we tested whether GluN2D-containing NMDARs participate in the physiological changes in the medial prefrontal cortex (mPFC) triggered by ketamine. Sub-anesthetic dose of ketamine (25 mg/kg) elevated the frequency of spontaneous excitatory postsynaptic currents (sEPSC) in wild-type (WT) mice, but not in GluN2D KO mice, 1 h after the injection. The amplitude of sEPSC and paired-pulse ratio (PPR) were unaltered by ketamine in both WT and GluN2D KO mice. These findings suggest that GluN2D-containing NMDARs might play a role in the ketamine-mediated changes in glutamatergic neurons in mPFC and, presumably, in ketamine-induced hyperlocomotion.
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影响因子：
64.8
作者：
Vesuna S;Kauvar IV;Richman E;Gore F;Oskotsky T;Sava-Segal C;Luo L;Malenka RC;Henderson JM;Nuyujukian P;Parvizi J;Deisseroth K
通讯作者：
Deisseroth K
DOI：
10.1126/science.1190287
发表时间：
2010-08-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li N;Lee B;Liu RJ;Banasr M;Dwyer JM;Iwata M;Li XY;Aghajanian G;Duman RS
通讯作者：
Duman RS
影响因子：
7.7
作者：
Miller OH;Yang L;Wang CC;Hargroder EA;Zhang Y;Delpire E;Hall BJ
通讯作者：
Hall BJ
影响因子：
3.7
作者：
Hagino Y;Kasai S;Han W;Yamamoto H;Nabeshima T;Mishina M;Ikeda K
通讯作者：
Ikeda K
影响因子：
3.7
作者：
Garst-Orozco, Jonathan;Malik, Ruchi;Buhl, Derek L.
通讯作者：
Buhl, Derek L.