PACAP enhances axon outgrowth in cultured hippocampal neurons to a comparable extent as BDNF.
PACAP enhances axon outgrowth in cultured hippocampal neurons to a comparable extent as BDNF.
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DOI：
10.1371/journal.pone.0120526
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hashimoto H
中科院分区：
文献类型：
--
作者：
Ogata K;Shintani N;Hayata-Takano A;Kamo T;Higashi S;Seiriki K;Momosaki H;Vaudry D;Vaudry H;Galas L;Kasai A;Nagayasu K;Nakazawa T;Hashimoto R;Ago Y;Matsuda T;Baba A;Hashimoto H
腺苷酸环化酶激活多肽（PACAP）具有神经营养活性，包括调节突触可塑性和记忆、海马神经发生和神经保护，其中大部分与脑源性神经营养因子（BDNF）共享。因此，本研究的目的是比较PACAP和BDNF对原代培养海马神经元的形态学影响。在体外（DIV）3天，PACAP增加神经突长度和数量到与BDNF相似的水平，但血管活性肠多肽显示出低得多的效果。此外，PACAP增加轴突，但不是树突，长度和索马大小在DIV 3类似于BDNF。PACAP拮抗剂PACAP 6 -38完全阻断了PACAP诱导的轴突长度增加，但不阻断树突长度增加。有趣的是，BDNF诱导的轴突长度增加也被PACAP 6 -38抑制，这表明PACAP信号转导机制。TrkB受体抑制剂K252 a抑制PACAP和BDNF诱导的轴突生长，而不影响树突长度。这些结果表明，在原代培养的海马神经元中，PACAP通过其同源受体PAC 1表现出形态学作用，刺激神经突长度和数量以及索马大小达到与BDNF相当的程度，并且总神经突长度的增加归因于轴突生长。
Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts neurotrophic activities including modulation of synaptic plasticity and memory, hippocampal neurogenesis, and neuroprotection, most of which are shared with brain-derived neurotrophic factor (BDNF). Therefore, the aim of this study was to compare morphological effects of PACAP and BDNF on primary cultured hippocampal neurons. At days in vitro (DIV) 3, PACAP increased neurite length and number to similar levels by BDNF, but vasoactive intestinal polypeptide showed much lower effects. In addition, PACAP increased axon, but not dendrite, length, and soma size at DIV 3 similarly to BDNF. The PACAP antagonist PACAP6–38 completely blocked the PACAP-induced increase in axon, but not dendrite, length. Interestingly, the BDNF-induced increase in axon length was also inhibited by PACAP6–38, suggesting a mechanism involving PACAP signaling. K252a, a TrkB receptor inhibitor, inhibited axon outgrowth induced by PACAP and BDNF without affecting dendrite length. These results indicate that in primary cultured hippocampal neurons, PACAP shows morphological actions via its cognate receptor PAC1, stimulating neurite length and number, and soma size to a comparable extent as BDNF, and that the increase in total neurite length is ascribed to axon outgrowth.
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影响因子：
15.9
作者：
Freson, K;Hashimoto, H;Van Geet, C
通讯作者：
Van Geet, C
DOI：
10.1007/s12031-012-9818-1
发表时间：
2012-11
期刊：
Journal of molecular neuroscience : MN
影响因子：
--
作者：
Lazarovici P;Cohen G;Arien-Zakay H;Chen J;Zhang C;Chopp M;Jiang H
通讯作者：
Jiang H
影响因子：
11
作者：
Hashimoto, R.;Hashimoto, H.;Baba, A.
通讯作者：
Baba, A.
DOI：
10.1016/s0165-3806(99)00192-3
发表时间：
2000-03-15
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Jaworski, DM;Proctor, MD
通讯作者：
Proctor, MD
影响因子：
2.5
作者：
Dong, Ying Xu;Fukuchi, Mamoru;Tsuda, Masaaki
通讯作者：
Tsuda, Masaaki