Protective Effect of Highly Polymeric A-Type Proanthocyanidins from Seed Shells of Japanese Horse Chestnut (Aesculus turbinata BLUME) against Light-Induced Oxidative Damage in Rat Retina.
Protective Effect of Highly Polymeric A-Type Proanthocyanidins from Seed Shells of Japanese Horse Chestnut (Aesculus turbinata BLUME) against Light-Induced Oxidative Damage in Rat Retina.
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DOI：
10.3390/nu10050593
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发表时间：
2018-05-10
期刊：
影响因子：
5.9
通讯作者：
Ohira A
中科院分区：
文献类型：
--
作者：
Ishihara T;Kaidzu S;Kimura H;Koyama Y;Matsuoka Y;Ohira A
视网膜组织暴露于由可见光引起的氧化应激。在年龄相关性黄斑变性（AMD）研究中使用的光损伤大鼠阐明了抗氧化剂可减少视网膜光损伤。将白化病大鼠暴露于5000 Lux光下12 h，口服给予来自日本七叶树种子壳的多酚化合物级分（PF）（30 mg/kg、100 mg/kg和300 mg/kg体重：BW）。为了评估对光损伤的保护作用，检查视网膜电图（ERG）、外核层（ONL）厚度、血浆抗氧化活性、氧化视网膜脂质和细胞凋亡检测。为了揭示其活性成分，PF被分离成A型原花青素（PAF）和黄酮醇O-糖苷馏分。通过测量ERG和ONL的厚度来比较这些组分对光损伤的保护作用。与阴性对照组相比，PF组（100 mg/kg和300 mg/kg BW）显著抑制ERG振幅和ONL厚度的降低。PF（300 mg/kg BW）诱导体内抗氧化活性升高，并抑制视网膜脂质氧化。PF管理也抑制凋亡细胞死亡。PAF对光损伤的保护作用与其抗氧化活性有关。口服PF和PAF对视网膜光损伤有保护作用。总之，这些化合物对于预防由光暴露引起的疾病是潜在有用的。亮点：利用日本七叶树（Aesculus turbinata BLUME）种子壳中的多酚类化合物作为抗氧化剂，评价了光暴露对视网膜损伤的保护作用。降低视网膜电图振幅和外核层厚度被抑制的多酚化合物的种子壳。日本七叶树种子壳中的多酚类化合物抑制视网膜脂质的氧化。高度聚合的A型原花青素从种子壳保护大鼠视网膜从光暴露损伤，抑制氧化应激和凋亡机制。
Retinal tissue is exposed to oxidative stress caused by visible light. Light-damaged rat used in age-related macular degeneration (AMD) studies clarified that antioxidants decrease retinal light damage. Albino rats were exposed to 5000 Lux light for 12 h with oral administration of the polyphenolic compounds fraction (PF) from the seed shells of Japanese horse chestnut (30 mg/kg, 100 mg/kg, and 300 mg/kg body weight: BW). To evaluate the protective effects against light damage, electroretinograms (ERGs), the outer nuclear layer (ONL) thickness, the antioxidant activity of plasma, oxidized retinal lipids, and the detection of apoptosis were examined. To reveal their active compounds, PF were separated into an A-type proanthocyanidin (PAF) and a flavonol O-glycosides fraction. The protective effects of these fractions against light damage were compared by measuring the thickness of the ERGs and ONL. Compared with the negative control, the PF group (100 mg/kg and 300 mg/kg BW) significantly suppressed the decrease of the ERG amplitudes and ONL thickness. PF (300 mg/kg BW) induced the elevation of in vivo antioxidant activity, and the suppression of retinal lipid oxidation. PF administration also suppressed apoptotic cell death. The protective effects against light damage were attributable to the antioxidant activity of PAF. The light-induced damage of retinas was protected by oral administration of PF and PAF. Taken together, these compounds are potentially useful for the prevention of the disease caused by light exposure. Highlights: The protective effects of retinal damage by light exposure were evaluated using polyphenolic compounds from the seed shells of Japanese horse chestnut (Aesculus turbinata BLUME) as an antioxidant. Decreases in the electroretinographic amplitude and outer nuclear layer thickness were suppressed by the polyphenolic compounds of the seed shells. Polyphenolic compounds from the seed shells of Japanese horse chestnut inhibited the oxidation of retinal lipids. Highly polymeric A-type proanthocyanidin from the seed shells protected the rat retina from light exposure damage by inhibiting oxidative stress and apoptotic mechanisms.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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