Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results.
Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results.
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DOI：
10.1186/1742-2094-9-222
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发表时间：
2012-09-21
影响因子：
9.3
通讯作者：
Hashimoto T
中科院分区：
文献类型：
--
作者：
Hasan D;Chalouhi N;Jabbour P;Hashimoto T
M1和M2细胞是人类巨噬细胞的两个主要亚群，对炎症反应产生相反的作用。本研究旨在探讨巨噬细胞M1/M2失衡和肥大细胞在人类脑动脉瘤破裂过程中的作用。10例脑动脉瘤患者（5例破裂和5例未破裂）接受了显微手术夹闭。在手术过程中，切除一段动脉瘤圆顶，并用M1细胞（抗HLA DR）、M2细胞（抗CD 163）和肥大细胞（抗类胰蛋白酶克隆AA）的单克隆抗体进行免疫染色。还取出一段颞浅动脉（STA），并用M1、M2和肥大细胞的单克隆抗体进行免疫染色。所有10个动脉瘤组织的M1、M2和肥大细胞染色均呈阳性。在未破裂的动脉瘤中，M1和M2细胞的比例相等。这与破裂动脉瘤中M1细胞明显多于M2细胞形成对比（p = 0.045）。肥大细胞在破裂的动脉瘤中也显著上调（p = 0.001）。STA样品中存在少量M1和M2细胞。M1/M2巨噬细胞和肥大细胞在人类脑动脉瘤中发现;然而，M1和肥大细胞表达似乎在破裂的动脉瘤中显著增加。这些发现表明，巨噬细胞M1/M2失衡和肥大细胞的上调可能在脑动脉瘤破裂的进展中发挥作用。
M1 and M2 cells are two major subsets of human macrophages that exert opposite effects on the inflammatory response. This study aims to investigate the role of macrophage M1/M2 imbalance and mast cells in the progression of human cerebral aneurysms to rupture. Ten patients with cerebral aneurysms (five ruptured and five unruptured) underwent microsurgical clipping. During the procedure, a segment of the aneurysm dome was resected and immunostained with monoclonal antibodies for M1 cells (anti-HLA DR), M2 cells (anti-CD 163), and mast cells (anti-tryptase clone AA). A segment of the superficial temporal artery (STA) was also removed and immunostained with monoclonal antibodies for M1, M2, and mast cells. All ten aneurysm tissues stained positive for M1, M2, and mast cells. M1 and M2 cells were present in equal proportions in unruptured aneurysms. This contrasted with a marked predominance of M1 over M2 cells in ruptured aneurysms (p = 0.045). Mast cells were also prominently upregulated in ruptured aneurysms (p = 0.001). Few M1 and M2 cells were present in STA samples. M1/M2 macrophages and mast cells are found in human cerebral aneurysms; however, M1 and mast cell expression seems to markedly increase in ruptured aneurysms. These findings suggest that macrophage M1/M2 imbalance and upregulation of mast cells may have a role in the progression of cerebral aneurysms to rupture.
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影响因子：
4.6
作者：
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