A wheat chromosome segment substitution line series supports characterisation and use of progenitor genetic variation

A wheat chromosome segment substitution line series supports characterisation and use of progenitor genetic variation
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小麦染色体片段替换系系列支持祖遗传变异的表征和使用

DOI:
10.1101/2022.06.18.496684
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发表时间:
2022
期刊:
--
影响因子:
--
通讯作者:
Horsnell R
Horsnell R
中科院分区:
--
文献类型:
--
作者:
Horsnell R

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在许多植物物种中已经开发了全基因组渐渗和置换系,提高了作图精度、基因发现以及鉴定和利用来自野生近缘的变异。通过多代杂交和/或回交伴随着标记辅助选择而产生,所得的渐渗系是固定的遗传资源。本文报道了春小麦(Triticum aestivumL.)染色体片段置换系(CSSLs)产生以系统地捕获来自四倍体(T. turgidumssp.dicoccoides)和二倍体粗山羊草(Aegilops tauschii)的祖先物种。在一个共同的遗传背景下产生的四个世代的回交,这是一个基础资源的定位和表征小麦祖先的变化。为了便于进一步开发,使用高密度基因分型阵列对最终群体进行遗传表征,并评估一系列农艺和谷物性状,以证明群体在小麦中用于性状定位的潜在用途。
Genome‐wide introgression and substitution lines have been developed in many plant species, enhancing mapping precision, gene discovery, and the identification and exploitation of variation from wild relatives. Created over multiple generations of crossing and/or backcrossing accompanied by marker‐assisted selection, the resulting introgression lines are a fixed genetic resource. In this study we report the development of spring wheat (Triticum aestivumL.) chromosome segment substitution lines (CSSLs) generated to systematically capture genetic variation from tetraploid (T. turgidumssp.dicoccoides) and diploid (Aegilops tauschii) progenitor species. Generated in a common genetic background over four generations of backcrossing, this is a base resource for the mapping and characterization of wheat progenitor variation. To facilitate further exploitation the final population was genetically characterized using a high‐density genotyping array and a range of agronomic and grain traits assessed to demonstrate the potential use of the populations for trait localization in wheat.
DOI: 10.1105/tpc.113.112391
发表时间: 2013-07-01
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影响因子: 11.6
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