Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility.
Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility.
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DOI：
10.1186/1477-7827-7-55
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发表时间：
2009-05-29
期刊：
影响因子：
--
通讯作者：
Sheldon IM
中科院分区：
文献类型：
--
作者：
Herath S;Lilly ST;Santos NR;Gilbert RO;Goetze L;Bryant CE;White JO;Cronin J;Sheldon IM
牛分娩后子宫腔细菌污染普遍存在。有些动物会患子宫炎，生育能力下降，但其他动物没有子宫疾病，很容易怀孕。本研究验证了以下假设，即产后发生持续性子宫炎和不孕症的牛无法限制对子宫细菌感染的炎症反应。在产后期间，从随后不育伴持续性子宫内膜炎（n = 4）或无临床疾病且首次授精时受孕（n = 4）的动物中多次采集子宫内膜活检。采用定量PCR检测子宫内膜活检组织中候选基因的表达，包括先天免疫受体Toll样受体（TLR 1至10）家族、炎症介质及其同源受体。通过免疫组织化学检查选定的蛋白质。编码促炎介质如白细胞介素（IL 1A，IL 1B和IL 6）和一氧化氮合酶2（NOS 2）的基因的表达在产后第一周高于随后。在产后第一周，不育动物的TLR4（细菌脂多糖受体）、促炎细胞因子IL1A和IL1B及其受体IL1R2的基因表达高于生育动物。编码其他Toll样受体、转化生长因子β受体1（TGFBR 1）或前列腺素E2受体（PTGER 2和PTGER 4）的基因表达在动物组之间没有显著差异。产后第一周后，不育和生育动物的基因表达没有显着差异。然而，有较高的比率IL 1A或IL 1B mRNA的抗炎细胞因子IL 10，在产后第一周不育的动物比生育的动物，这些基因的蛋白产物主要定位于子宫内膜的上皮。牛在分娩后第一周内，可通过限制子宫内膜对产后细菌感染的炎症反应来维持生育力。
Contamination of the uterine lumen with bacteria is ubiquitous in cattle after parturition. Some animals develop endometritis and have reduced fertility but others have no uterine disease and readily conceive. The present study tested the hypothesis that postpartum cattle that develop persistent endometritis and infertility are unable to limit the inflammatory response to uterine bacterial infection. Endometrial biopsies were collected several times during the postpartum period from animals that were subsequently infertile with persistent endometritis (n = 4) or had no clinical disease and conceived to first insemination (n = 4). Quantitative PCR was used to determine the expression of candidate genes in the endometrial biopsies, including the Toll-like receptor (TLR 1 to 10) family of innate immune receptors, inflammatory mediators and their cognate receptors. Selected proteins were examined by immunohistochemistry. The expression of genes encoding pro-inflammatory mediators such as interleukins (IL1A, IL1B and IL6), and nitric oxide synthase 2 (NOS2) were higher during the first week post partum than subsequently. During the first week post partum, there was higher gene expression in infertile than fertile animals of TLR4, the receptor for bacterial lipopolysaccharide, and the pro-inflammatory cytokines IL1A and IL1B, and their receptor IL1R2. The expression of genes encoding other Toll-like receptors, transforming growth factor beta receptor 1 (TGFBR1) or prostaglandin E2 receptors (PTGER2 and PTGER4) did not differ significantly between the animal groups. Gene expression did not differ significantly between infertile and fertile animals after the first week postpartum. However, there were higher ratios of IL1A or IL1B mRNA to the anti-inflammatory cytokine IL10, during the first week post partum in the infertile than fertile animals, and the protein products of these genes were mainly localised to the epithelium of the endometrium. Cattle may maintain fertility by limiting the inflammatory response to postpartum bacterial infection in the endometrium during the first week after parturition.
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