Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices.
Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices.
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DOI：
10.1016/j.xpro.2021.100442
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Grueter BA
中科院分区：
文献类型：
--
作者：
Manz KM;Siemann JK;McMahon DG;Grueter BA
膜片钳和多电极阵列电生理技术被用来测量神经元的动态功能特性。全细胞和细胞贴附膜片钳记录可以在电压钳和电流钳配置中进行，以揭示控制神经传递的细胞类型特异性突触和细胞参数。多电极阵列电生理学可以提供来自多个神经元的尖峰活动记录，从而实现更大的样本量和长期记录。我们提供了我们的指南，准备急性啮齿动物脑切片与示例实验和分析，旨在为新手和专家电生理学家。有关本方案使用和执行的完整详细信息，请参阅。可行和有效的小鼠脑组织制备急性脑切片电生理学的全面材料来源神经元全细胞膜片钳记录的详细方法多电极阵列电生理学在小鼠脑中的用途和应用膜片钳和多电极阵列电生理技术用于测量神经元的动态功能特性。全细胞和细胞贴附膜片钳记录可以在电压钳和电流钳配置中进行，以揭示控制神经传递的细胞类型特异性突触和细胞参数。多电极阵列电生理学可以提供多个神经元的锋电位活动记录，从而实现更大的样本量和长期记录。我们提供了我们的指南，准备急性啮齿动物脑切片与示例实验和分析，旨在为新手和专家电生理学家。
Patch-clamp and multi-electrode array electrophysiology techniques are used to measure dynamic functional properties of neurons. Whole-cell and cell-attached patch-clamp recordings in brain slices can be performed in voltage-clamp and current-clamp configuration to reveal cell-type-specific synaptic and cellular parameters governing neurotransmission. Multi-electrode array electrophysiology can provide spike activity recordings from multiple neurons, enabling larger sample sizes, and long-term recordings. We provide our guide to preparing acute rodent brain slices with example experiments and analyses intended for novice and expert electrophysiologists. For complete details on the use and execution of this protocol, please refer to. Viable and efficient preparation of mouse brain tissue Comprehensive material source for acute brain slice electrophysiology Detailed approach to whole-cell patch-clamp recording of neurons Utility and application of multi-electrode array electrophysiology in mouse brain Patch-clamp and multi-electrode array electrophysiology techniques are used to measure dynamic functional properties of neurons. Whole-cell and cell-attached patch-clamp recordings in brain slices can be performed in voltage-clamp and current-clamp configuration to reveal cell-type-specific synaptic and cellular parameters governing neurotransmission. Multi-electrode array electrophysiology can provide spike activity recordings from multiple neurons, enabling larger sample sizes, and long-term recordings. We provide our guide to preparing acute rodent brain slices with example experiments and analyses intended for novice and expert electrophysiologists.
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影响因子：
10.3
作者：
Giannoni-Guzmán MA;Kamitakahara A;Magalong V;Levitt P;McMahon DG
通讯作者：
McMahon DG
影响因子：
3.4
作者：
MATHIAS, RT;COHEN, IS;OLIVA, C
通讯作者：
OLIVA, C
影响因子：
5.3
作者：
Manz, Kevin M.;Baxley, Andrew G.;Grueter, Brad A.
通讯作者：
Grueter, Brad A.
影响因子：
64.8
作者：
MALINOW, R;TSIEN, RW
通讯作者：
TSIEN, RW
影响因子：
2.9
作者：
Herzog, ED;Geusz, ME;Block, GD
通讯作者：
Block, GD