Exercise‐Induced Changes in Plasma Composition Increase Erythrocyte Na+,K+‐ATPase, but not Na+–K+–2Cl− Cotransporter, Activity to Stimulate net and Unidirectional K+ Transport in Humans
Exercise‐Induced Changes in Plasma Composition Increase Erythrocyte Na+,K+‐ATPase, but not Na+–K+–2Cl− Cotransporter, Activity to Stimulate net and Unidirectional K+ Transport in Humans
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运动引起的血浆成分变化会增加红细胞 Na+、K+-ATP 酶，但不会增加 Na+–K+–2Cl− 协同转运蛋白、刺激人体净和单向 K+ 转运的活性
DOI：
10.1113/jphysiol.2003.052860
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. P. Grudzien
中科院分区：
文献类型：
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作者：
M. Lindinger;S. P. Grudzien
We tested the hypothesis that exercise‐induced changes in plasma composition result in peak stimulation of erythrocyte unidirectional K+ (JK,in) and net K+ (JK,net) transport within the first 120 s. In experimental series 1 (7 men; 2 women), plasma [K+] was continuously measured in vitro (37 °C) after the addition of red blood cells (RBCs) obtained from rested subjects (resting RBCs) into an exercise‐simulated plasma (ESP; increased plasma osmolality, [K+], [H+], [lactate] and [adrenaline] (epinephrine)), and JK,net calculated. In experimental series 2 (7 men; 4 women), resting RBCs were incubated in true exercise plasma (TEP) obtained after two 30 s bouts of high intensity leg cycling exercise to determine JK,net and JK,in (via RBC 86Rb accumulation). JK,net of resting RBCs increased from 0.9 ± 28.7 in resting plasma to 285 ± 164 mmol (l RBCs)−1 h−1 in ESP and to 178 ± 60 mmol (l RBCs)−1 h−1 after 10 s in TEP. Both JK,net and JK,in peaked within 10 s of incubation and decreased rapidly during the initial 120 s. The use of inhibitors for the Na+,K+‐ATPase (ouabain) and the Na+‐K+‐2Cl− cotransporter (NKCC; bumetanide) indicated that rapid increases in JK,in and JK,net upon incubation of resting RBCs in TEP were due primarily to increased Na+,K+‐ATPase activity; the NKCC appeared to be involved only when the Na+,K+‐ATPase was blocked. It is concluded that RBCs rapidly increase JK,in and JK,net in response to exercise‐induced changes in plasma composition.
静水压力梯度对运动后血浆容量调节的影响。
DOI：
10.1152/jappl.1998.85.2.667
发表时间：
1998
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
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作者：
Mack,GW;Yang,R;Hargens,AR;Nagashima,K;Haskell,A
通讯作者：
Haskell,A