The E3 ligase MREL57 modulates microtubule stability and stomatal closure in response to ABA.
The E3 ligase MREL57 modulates microtubule stability and stomatal closure in response to ABA.
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E3 连接酶 MREL57 响应 ABA 调节微管稳定性和气孔关闭
DOI：
10.1038/s41467-021-22455-y
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发表时间：
2021-04-12
影响因子：
16.6
通讯作者：
Mao T
中科院分区：
文献类型：
--
作者：
Dou L;He K;Peng J;Wang X;Mao T
气孔运动的调节是植物适应环境胁迫的关键。脱落酸（阿坝）诱导气孔关闭时，微管骨架发生解体。然而，这种监管的机制在很大程度上仍不清楚。在这里，我们表明，泛素-26S蛋白酶体（UPS）依赖的途径介导微管拆卸，ABA诱导的气孔关闭所需的。此外，我们确定并表征泛素E3连接酶MIP 57（微管相关的E3连接酶57）和微管稳定蛋白WDL 7（WAVE-DAMPENED 2-LIKE 7）inclusionopsis和显示MIP 57-WDL 7模块调节微管解体介导气孔关闭响应干旱胁迫和阿坝处理。MSN 57与WDL 7相互作用，泛素化并降解WDL 7，并且这种作用明显被阿坝增强。ABA诱导的气孔关闭和微管解体在mrel 57突变体中被显著抑制，当WDL 7表达降低时，这些表型可以恢复。我们的研究结果揭示了依赖UPS的机制和MST 57-WDL 7模块在响应干旱胁迫和阿坝的微管解体和气孔关闭中的作用。
Regulation of stomatal movement is critical for plant adaptation to environmental stresses. The microtubule cytoskeleton undergoes disassembly, which is critical for stomatal closure in response to abscisic acid (ABA). However, the mechanism underlying this regulation largely remains unclear. Here we show that a ubiquitin-26S proteasome (UPS)-dependent pathway mediates microtubule disassembly and is required for ABA-induced stomatal closure. Moreover, we identify and characterize the ubiquitin E3 ligase MREL57 (MICROTUBULE-RELATED E3 LIGASE57) and the microtubule-stabilizing protein WDL7 (WAVE-DAMPENED2-LIKE7) inArabidopsisand show that the MREL57-WDL7 module regulates microtubule disassembly to mediate stomatal closure in response to drought stress and ABA treatment. MREL57 interacts with, ubiquitinates and degrades WDL7, and this effect is clearly enhanced by ABA. ABA-induced stomatal closure and microtubule disassembly are significantly suppressed inmrel57mutants, and these phenotypes can be restored whenWDL7expression is decreased. Our results unravel UPS-dependent mechanisms and the role of an MREL57-WDL7 module in microtubule disassembly and stomatal closure in response to drought stress and ABA.
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