Gene Duplication and Gene Expression Changes Play a Role in the Evolution of Candidate Pollen Feeding Genes in Heliconius Butterflies.
Gene Duplication and Gene Expression Changes Play a Role in the Evolution of Candidate Pollen Feeding Genes in Heliconius Butterflies.
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DOI：
10.1093/gbe/evw180
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发表时间：
2016-09-02
影响因子：
3.3
通讯作者：
Briscoe AD
中科院分区：
文献类型：
--
作者：
Smith G;Macias-Muñoz A;Briscoe AD
在蝴蝶中，Heliconius 拥有一种独特的以花粉为食的能力。花粉喂养显着延长了它们的寿命，并且被认为对该属的多样化很重要。我们使用 RNA 测序来检查四种 Heliconius 和一种非花粉摄食物种 Eueides isabella 口器中与取食相关的基因表达。我们假设，参与形态和蛋白质代谢的基因可能在Heliconius中上调，因为它们的长鼻比Eueides更长，而且花粉比花蜜含有更多的蛋白质。使用从头转录组组装，我们通过比较口器与触角和腿的基因表达来测试这些假设。我们首先在所有五个物种的口器中寻找上调的基因，并发现了数百个基因，其中许多具有涉及蛋白质（茧酶）、脂质和碳水化合物代谢的功能注释。然后，我们在 Heliconius 中进行了专门研究，发现了 11 种常见的上调基因，这些基因在形态（CPR 角质层蛋白）、行为（类似外卖）和新陈代谢（类似荧光素酶）中发挥作用。对这些候选者的仔细检查发现，茧酶沿着导致赫利康蝴蝶的谱系经历了几次重复，其中包括两次赫利康特有的重复。萤光素酶样基因也在鳞翅目动物中发生复制，并在Heliconius口器中发生上调。逆转录 PCR 证实，三种椰子酶、一种肽酶和一种荧光素酶样基因在喙中表达，而在唇须和唾液腺中表达很少或不表达。我们的研究结果表明，与其他饮食专门化一样，花粉喂养很可能是通过预先存在的基因的适应性扩展而促进的，并且蝴蝶的长鼻参与了消化酶的产生。
Heliconius possess a unique ability among butterflies to feed on pollen. Pollen feeding significantly extends their lifespan, and is thought to have been important to the diversification of the genus. We used RNA sequencing to examine feeding-related gene expression in the mouthparts of four species of Heliconius and one nonpollen feeding species, Eueides isabella. We hypothesized that genes involved in morphology and protein metabolism might be upregulated in Heliconius because they have longer proboscides than Eueides, and because pollen contains more protein than nectar. Using de novo transcriptome assemblies, we tested these hypotheses by comparing gene expression in mouthparts against antennae and legs. We first looked for genes upregulated in mouthparts across all five species and discovered several hundred genes, many of which had functional annotations involving metabolism of proteins (cocoonase), lipids, and carbohydrates. We then looked specifically within Heliconius where we found eleven common upregulated genes with roles in morphology (CPR cuticle proteins), behavior (takeout-like), and metabolism (luciferase-like). Closer examination of these candidates revealed that cocoonase underwent several duplications along the lineage leading to heliconiine butterflies, including two Heliconius-specific duplications. Luciferase-like genes also underwent duplication within lepidopterans, and upregulation in Heliconius mouthparts. Reverse-transcription PCR confirmed that three cocoonases, a peptidase, and one luciferase-like gene are expressed in the proboscis with little to no expression in labial palps and salivary glands. Our results suggest pollen feeding, like other dietary specializations, was likely facilitated by adaptive expansions of preexisting genes—and that the butterfly proboscis is involved in digestive enzyme production.
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影响因子：
3.8
作者：
Andersen, SO
通讯作者：
Andersen, SO
影响因子：
64.8
作者：
Dasmahapatra, Kanchon K.;Walters, James R.;Briscoe, Adriana D.;Davey, John W.;Whibley, Annabel;Nadeau, Nicola J.;Zimin, Aleksey V.;Hughes, Daniel S. T.;Ferguson, Laura C.;Martin, Simon H.;Salazar, Camilo;Lewis, James J.;Adler, Sebastian;Ahn, Seung-Joon;Baker, Dean A.;Baxter, Simon W.;Chamberlain, Nicola L.;Chauhan, Ritika;Counterman, Brian A.;Dalmay, Tamas;Gilbert, Lawrence E.;Gordon, Karl;Heckel, David G.;Hines, Heather M.;Hoff, Katharina J.;Holland, Peter W. H.;Jacquin-Joly, Emmanuelle;Jiggins, Francis M.;Jones, Robert T.;Kapan, Durrell D.;Kersey, Paul;Lamas, Gerardo;Lawson, Daniel;Mapleson, Daniel;Maroja, Luana S.;Martin, Arnaud;Moxon, Simon;Palmer, William J.;Papa, Riccardo;Papanicolaou, Alexie;Pauchet, Yannick;Ray, David A.;Rosser, Neil;Salzberg, Steven L.;Supple, Megan A.;Surridge, Alison;Tenger-Trolander, Ayse;Vogel, Heiko;Wilkinson, Paul A.;Wilson, Derek;Yorke, James A.;Yuan, Furong;Balmuth, Alexi L.;Eland, Cathlene;Gharbi, Karim;Thomson, Marian;Gibbs, Richard A.;Han, Yi;Jayaseelan, Joy C.;Kovar, Christie;Mathew, Tittu;Muzny, Donna M.;Ongeri, Fiona;Pu, Ling-Ling;Qu, Jiaxin;Thornton, Rebecca L.;Worley, Kim C.;Wu, Yuan-Qing;Linares, Mauricio;Blaxter, Mark L.;Ffrench-Constant, Richard H.;Joron, Mathieu;Kronforst, Marcus R.;Mullen, Sean P.;Reed, Robert D.;Scherer, Steven E.;Richards, Stephen;Mallet, James;McMillan, W. Owen;Jiggins, Chris D.
通讯作者：
Jiggins, Chris D.
DOI：
10.18632/aging.100146
发表时间：
2010-05
期刊：
Aging
影响因子：
--
作者：
Bauer J;Antosh M;Chang C;Schorl C;Kolli S;Neretti N;Helfand SL
通讯作者：
Helfand SL
影响因子：
4.6
作者：
Blekhman, Ran;Perry, George H.;Gilad, Yoav
通讯作者：
Gilad, Yoav
影响因子：
1
作者：
Dacks, AM;Nickel, T;Mitchell, BK
通讯作者：
Mitchell, BK