Inhibition of Saccharomyces boulardii (nom. inval.) on cell invasion of Salmonella typhimurium and Yersinia enterocolitica
Inhibition of Saccharomyces boulardii (nom. inval.) on cell invasion of Salmonella typhimurium and Yersinia enterocolitica
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布拉酵母菌（名义无效）对鼠伤寒沙门氏菌和小肠结肠炎耶尔森氏菌细胞侵袭的抑制作用
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Altwegg
中科院分区：
文献类型：
--
作者：
R. Zbinden;E. Gönczi;M. Altwegg
研究了酵母菌Saccharomyces boulardii（nom. inval.）对鼠伤寒沙门氏菌和小肠结肠炎耶尔森氏菌的作用。用1.4 × 10 7 /ml S.鼠伤寒沙门氏菌在5小时内生长至6.6 × 10 8 /ml，但如果与约10 8 /ml活S共孵育，则仅生长至1.9 × 10 8 /ml。布拉地。Y.小肠结肠炎菌在S.布拉地。此外，S. typhimurium和Y.在HeLa细胞培养物中测量小肠结肠炎。超过10%的S。鼠伤寒沙门氏菌和60%以上的Y.小肠结肠炎菌在3 h时可作为活的细胞内生物体回收。与S. boulardii，低于S. typhimurium和Y.小肠结肠炎菌，分别最初加入到单层，是细胞内的。这些体外实验表明，S。布拉氏藻不仅抑制S. typhimurium和Y.小肠结肠炎，但也侵入HeLa细胞。然而，S.布拉地用于治疗由这些细菌引起的腹泻仍有待于在体内显示。关键词：布拉酵母菌，细菌入侵，鼠伤寒沙门氏菌，小肠结肠炎耶尔森氏菌。
The influence of the biotherapeutic yeast Saccharomyces boulardii (nom. inval.) on Salmonella typhimurium and Yersinia enterocolitica was investigated in vitro . A suspension of 1.4x 10 7 /ml S. typhimurium grew to 6.6x 10 8 /ml within five hours but only to 1.9x 10 8 /ml if coincubated with approximately 10 8 /ml live S. boulardii . A similar suspension of Y. enterocolitica did not multiply at all in the presence of S. boulardii . Furthermore, the invasion of S. typhimurium and Y. enterocolitica was measured in HeLa cell cultures. More than 10% of S. typhimurium and more than 60% of Y. enterocolitica , respectively, could be recovered as viable intracellular organisms at 3 h. After coincubation with S. boulardii , less than 0.1% of S. typhimurium and Y. enterocolitica , respectively, initially added to the monolayers, were intracellular. These in vitro experiments showed that S. boulardii inhibited not only growth of S. typhimurium and Y. enterocolitica but also their invasion into HeLa cells. Nevertheless, the value of S. boulardii for treating diarrhoea caused by these bacteria remains to be shown in vivo . Keywords: Saccharomyces boulardii , bacterial invasion, Salmonella typhimurium , Yersinia enterocolitica .
DOI：
10.1073/pnas.86.16.6383
发表时间：
1989-08-01
影响因子：
11.1
作者：
GALAN, JE;CURTISS, R
通讯作者：
CURTISS, R