The interaction between miR160 and miR165/166 in the control of leaf development and drought tolerance in Arabidopsis
The interaction between miR160 and miR165/166 in the control of leaf development and drought tolerance in Arabidopsis
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miR160和miR165/166之间的相互作用在控制拟南芥叶片发育和耐旱性中的作用
DOI：
10.1038/s41598-019-39397-7
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发表时间：
2019-02
影响因子：
4.6
通讯作者：
Tang Guiliang
中科院分区：
文献类型：
--
作者：
Yang Tianxiao;Wang Yongyan;Teotia Sachin;Wang Zhaohui;Shi Chaonan;Sun Huwei;Gu Yiyou;Zhang Zhanhui;Tang Guiliang
MicroRNAs（miRNAs）是一类在植物发育和非生物胁迫中起重要作用的非编码RNA。迄今为止，研究主要集中在单个miRNA的作用，然而，少数人已经解决了多个miRNA之间的相互作用。本研究利用短串联靶模拟物（STTM）研究了miR 160和miR 165/166之间的相互作用和调控回路，以及miR 165/166对拟南芥叶片发育和耐旱性的影响。通过将STTM 160拟南芥与STTM 165/166拟南芥杂交，我们成功地产生了miR 160和miR 165/166的双突变体。双突变体植物表现出一系列的妥协表型在叶片发育和耐旱性相比，在单STTM线的表型改变。RNA-seq和qRT-PCR分析表明，与两个单突变体相比，STTM指导的双突变体中生长素和阿坝信号传导基因的表达水平受到损害。我们的研究结果还表明，miR 160指导的生长素反应因子（ARF）的调节有助于通过生长素信号基因的叶片发育，而miR 165/166介导的HD-ZIP III调控通过阿坝信号赋予耐旱性。我们的研究进一步表明，ARF和HD-ZIP III可能在调节叶片发育和耐旱性方面发挥相反的作用，可以进一步应用于其他作物的农艺性状改良。
MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development and abiotic stresses. To date, studies have mainly focused on the roles of individual miRNAs, however, a few have addressed the interactions among multiple miRNAs. In this study, we investigated the interplay and regulatory circuit between miR160 and miR165/166 and its effect on leaf development and drought tolerance in Arabidopsis using Short Tandem Target Mimic (STTM). By crossing STTM160 Arabidopsis with STTM165/166, we successfully generated a double mutant of miR160 and miR165/166. The double mutant plants exhibited a series of compromised phenotypes in leaf development and drought tolerance in comparison to phenotypic alterations in the single STTM lines. RNA-seq and qRT-PCR analyses suggested that the expression levels of auxin and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two single mutants. Our results also suggested that miR160-directed regulation of auxin response factors (ARFs) contribute to leaf development via auxin signaling genes, whereas miR165/166- mediated HD-ZIP IIIs regulation confers drought tolerance through ABA signaling. Our studies further indicated that ARFs and HD-ZIP IIIs may play opposite roles in the regulation of leaf development and drought tolerance that can be further applied to other crops for agronomic traits improvement.
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