Suppression of microRNA159 impacts multiple agronomic traits in rice (Oryza sativa L.).
Suppression of microRNA159 impacts multiple agronomic traits in rice (Oryza sativa L.).
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MicroRNA159 的抑制影响水稻 (Oryza sativa L.) 的多种农艺性状
DOI：
10.1186/s12870-017-1171-7
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发表时间：
2017-11-21
影响因子：
5.3
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Zhao Y;Wen H;Teotia S;Du Y;Zhang J;Li J;Sun H;Tang G;Peng T;Zhao Q
背景：微小RNA（miRNA）是植物生长发育的重要调节因子。 miR159是不同植物物种之间保守的miRNA，在植物中具有多种功能。对miR159的研究主要是在模式植物拟南芥上进行的。在水稻中，对 miR159 的研究要么基于全基因组表达分析，重点关注对不同氮形式和非生物胁迫的反应，要么基于过度表达其前体的转基因植物的表型研究。 STTM（Short Tandem Target Mimic）是阻断植物内源成熟miRNA活性的有效工具。因此，可以通过STTM下调miR159来探索miR159在水稻中的具体作用。结果：本研究中，STTM成功抑制了成熟miR159的表达，导致其两个靶基因OsGAMYB和OsGAMYBL1（GAMYB-LIKE 1）的表达增加。总体而言，STTM159植物表现出身材矮小，器官尺寸较小，茎直径、旗叶长度、主圆锥花序、小穗壳和籽粒尺寸减小。茎、叶和成熟小穗壳的组织学分析显示，小维管束（SVB）数量减少，两个大脉（LV）之间的小脉（SV）数量减少，外薄壁细胞数量减少。对野生型植物和 STTM159 转基因植物之间差异表达基因的基因本体论 (GO) 富集分析表明，参与细胞分裂、生长素、细胞分裂素 (CK) 和油菜素类固醇 (BRs) 生物合成和信号传导的基因在 STTM159 植物中显着下调。结论：我们的数据表明，在水稻中，miR159 正向调节器官大小，包括 由于细胞分裂的促进，茎、叶和籽粒变大。 RNA-seq数据的进一步分析表明，STTM159转基因植物中细胞分裂的减少可能至少部分是由于参与细胞周期和激素稳态的基因表达较低所致，这为水稻miR159特异性功能提供了新的见解。
Background:microRNAs (miRNAs) are important regulators in plant growth and development. miR159 is a conserved miRNA among different plant species and has various functions in plants. Studies on miR159 are mostly done on model plant, Arabidopsis thaliana. In rice, studies on miR159 were either based upon genome-wide expression analyses focused upon responses to different nitrogen forms and abiotic stress or upon phenotypic studies of transgenic plants overexpressing its precursor. STTM (Short Tandem Target Mimic) is an effective tool to block the activity of endogenous mature miRNA activity in plant. Therefore, specific roles of miR159 in rice could be explored by down regulating miR159 through STTM.Results:In this study, expression of mature miR159 was successfully suppressed by STTM which resulted in the increased expressions of its two targets genes, OsGAMYB and OsGAMYBL1 (GAMYB-LIKE 1). Overall, STTM159 plants exhibited short stature along with smaller organ size and reduction in stem diameter, length of flag leaf, main panicle, spikelet hulls and grain size. Histological analysis of stem, leaf and mature spikelet hull showed the reduced number of small vascular bundles (SVB), less number of small veins (SV) between two big veins (LV) and less cell number in outer parenchyma. Gene Ontology (GO) enrichment analysis of differentially expressed genes between wild type plants and STTM159 transgenic plants showed that genes involved in cell division, auxin, cytokinin (CK) and brassinosteroids (BRs) biosynthesis and signaling are significantly down-regulated in STTM159 plants.Conclusion:Our data suggests that in rice, miR159 positively regulates organ size, including stem, leaf, and grain size due to the promotion of cell division. Further analysis from the RNA-seq data showed that the decreased cell divisions in STTM159 transgenic plants may result, at least partly from the lower expression of the genes involved in cell cycle and hormone homeostasis, which provides new insights of rice miR159-specific functions.
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影响因子：
18
作者：
Gao, Feng;Wang, Kun;Li, Shaoqing
通讯作者：
Li, Shaoqing
影响因子：
7.4
作者：
Alonso-Peral, Maria M.;Li, Junyan;Millar, Anthony A.
通讯作者：
Millar, Anthony A.
影响因子：
5.1
作者：
Guo, Jing;Song, Jian;Zhang, Xian Sheng
通讯作者：
Zhang, Xian Sheng
影响因子：
11.4
作者：
HEMERLY, A;ENGLER, JD;FERREIRA, P
通讯作者：
FERREIRA, P
影响因子：
4.6
作者：
Huang J;Li Z;Zhao D
通讯作者：
Zhao D