Disruption of the gene encoding the cell adhesion molecule DdCAD-1 leads to aberrant cell sorting and cell-type proportioning during Dictyostelium development.
Disruption of the gene encoding the cell adhesion molecule DdCAD-1 leads to aberrant cell sorting and cell-type proportioning during Dictyostelium development.
复制标题
编码细胞粘附分子 DdCAD-1 的基因被破坏会导致盘基网柄菌发育过程中细胞分选和细胞类型比例异常。
作者：
Estella Wong;Chunzhong Yang;Jun Wang;D. Fuller;W. Loomis;C. Siu
在Dictyosteoblastoma中的cadA基因编码Ca 2+依赖性细胞粘附分子DdCAD-1，其在发育开始后不久表达。为了研究DdCAD-1的生物学作用，通过同源重组破坏cadA基因。cadA无效细胞显示EDTA敏感细胞粘附减少50%。剩余的EDTA敏感的粘附位点对抗DdCAD-1抗体的解离具有抗性，表明它们是不同的粘附位点。缺乏DdCAD-1的细胞能够完成发育并形成子实体。然而，它们显示出异常的蛞蝓形态，并且达到顶点延迟了约6小时。孢子产量减少约50%。cadA（-）蛞蝓中前柄细胞的比例比亲本菌株增加了2.5倍。当用pcotB：：GFP转染cadA（-）细胞以标记前孢子细胞时，蛞蝓中异常的细胞分选模式变得明显。当突变前柄细胞与野生型前孢子细胞混合时，突变前柄细胞不能返回嵌合蛞蝓的前部位置，表明分选机制存在缺陷。当用cadA表达载体转染cadA（-）细胞时，野生型表型恢复。这些结果表明，除了细胞-细胞粘附，DdCAD-1在细胞类型比例和图案形成中起作用。
The cadA gene in Dictyostelium encodes the Ca2+-dependent cell adhesion molecule DdCAD-1, which is expressed soon after the initiation of development. To investigate the biological role of DdCAD-1, the cadA gene was disrupted by homologous recombination. The cadA-null cells showed a 50% reduction in EDTA-sensitive cell adhesion. The remaining EDTA-sensitive adhesion sites were resistant to dissociation by anti-DdCAD-1 antibody, suggesting that they were distinct adhesion sites. Cells that lacked DdCAD-1 were able to complete development and form fruiting bodies. However, they displayed abnormal slug morphology and culmination was delayed by approximately 6 hours. The yield of spores was reduced by approximately 50%. The proportion of prestalk cells in cadA(-) slugs showed a 2.5-fold increase over the parental strain. When cadA(-) cells were transfected with pcotB::GFP to label prespore cells, aberrant cell-sorting patterns in slugs became apparent. When mutant prestalk cells were mixed with wild-type prespore cells, mutant prestalk cells were unable to return to the anterior position of chimeric slugs, suggesting defects in the sorting mechanism. The wild-type phenotype was restored when cadA(-) cells were transfected with a cadA-expression vector. These results indicate that, in addition to cell-cell adhesion, DdCAD-1 plays a role in cell type proportioning and pattern formation.
盘基网柄菌中初始细胞类型的分选取决于tipA 基因。
DOI：
10.1006/dbio.1997.8538
发表时间：
1997
期刊：
Developmental biology.
影响因子：
--
作者：
Stege,JT;Shaulsky,G;Loomis,WF
通讯作者：
Loomis,WF
表达水母脱辅基水母发光蛋白的盘基网柄菌多细胞阶段的游离钙模式。
DOI：
10.1242/dev.121.8.2291
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Cubitt,AB;Firtel,RA;Fischer,G;Jaffe,LF;Miller,AL
通讯作者：
Miller,AL
盘基网柄菌中的丘细胞运动和形态发生。
DOI：
10.1006/dbio.1999.9208
发表时间：
1999
期刊：
Developmental biology.
影响因子：
--
作者：
Kellerman,KA;McNally,JG
通讯作者：
McNally,JG