Self-Incompatibility in Brassicaceae: Identification and Characterization of SRK-Like Sequences Linked to the S-Locus in the Tribe Biscutelleae
Self-Incompatibility in Brassicaceae: Identification and Characterization of SRK-Like Sequences Linked to the S-Locus in the Tribe Biscutelleae
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十字花科的自交不亲和性：与 Biscutelleae 部落中 S 基因座相关的 SRK 样序列的鉴定和表征
DOI：
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
X. Vekemans
中科院分区：
文献类型：
--
作者：
Jean;C. C. Gosset;R. Gries;Kevin Calin;É. Schmitt;V. Castric;X. Vekemans
自交不亲和性（SI）是许多被子植物中阻止自花受精的遗传系统。虽然来自菊科的植物呈现出明显独特的SI系统，该系统是该科的祖先，但在负责SI的S-位点的研究主要限于两个不同的谱系（分别为芸苔属和拟南芥-荠菜属）。在这里，我们调查SI在第三个深分支谱系的菊科：部落Biscutelleae。通过偶联测序的SI基因负责花粉识别（SRK）与表型分析的基础上控制授粉，我们确定了20个SRK样序列功能性连锁的13个S-单倍型在21个个体的Biscutella neustriaca和220幼苗。我们发现了两个遗传和系统发育特征的SI在Biscutelleae中，从参考拟南芥分支中观察到的模式：（1）SRK样序列簇成两个主要的系统发育谱系内散布在许多SRK谱系的拟南芥;和（2）一些SRK样序列传递的连锁对，表明本地重复的S-位点。引人注目的是，这些特征也在芸苔属分支中观察到，但可能是独立进化的，因为Biscutella中的两个主要SRK簇与芸苔属中的SRK簇不同。根据我们的研究结果和什么以前已经观察到在其他菊科植物，我们讨论了SI植物种群的高多样性和复杂的优势关系，我们发现在S-位点Biscutelleae的生态和进化的影响。
Self-incompatibility (SI) is a genetic system that prevents self-fertilization in many Angiosperms. Although plants from the Brassicaceae family present an apparently unique SI system that is ancestral to the family, investigations at the S-locus responsible for SI have been mostly limited to two distinct lineages (Brassica and Arabidopsis-Capsella, respectively). Here, we investigated SI in a third deep-branching lineage of Brassicaceae: the tribe Biscutelleae. By coupling sequencing of the SI gene responsible for pollen recognition (SRK) with phenotypic analyses based on controlled pollinations, we identified 20 SRK-like sequences functionally linked to 13 S-haplotypes in 21 individuals of Biscutella neustriaca and 220 seedlings. We found two genetic and phylogenetic features of SI in Biscutelleae that depart from patterns observed in the reference Arabidopsis clade: (1) SRK-like sequences cluster into two main phylogenetic lineages interspersed within the many SRK lineages of Arabidopsis; and (2) some SRK-like sequences are transmitted by linked pairs, suggesting local duplication within the S-locus. Strikingly, these features also were observed in the Brassica clade but probably evolved independently, as the two main SRK clusters in Biscutella are distinct from those in Brassica. In the light of our results and of what has been previously observed in other Brassicaceae, we discuss the ecological and evolutionary implications on SI plant populations of the high diversity and the complex dominance relationships we found at the S-locus in Biscutelleae.
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影响因子：
7.4
作者：
Guo, Ya-Long;Zhao, Xuan;Weigel, Detlef
通讯作者：
Weigel, Detlef
影响因子：
56.9
作者：
Schopfer, CR;Nasrallah, ME;Nasrallah, JB
通讯作者：
Nasrallah, JB
影响因子：
30.8
作者：
通讯作者：
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DOI：
10.1073/pnas.0808012106
发表时间：
2009-03-31
影响因子：
11.1
作者：
Guo, Ya-Long;Bechsgaard, Jesper S.;Schierup, Mikkel H.
通讯作者：
Schierup, Mikkel H.