Photoperiodic diapause under the control of circadian clock genes in an insect.
Photoperiodic diapause under the control of circadian clock genes in an insect.
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DOI：
10.1186/1741-7007-8-116
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发表时间：
2010-09-03
期刊：
影响因子：
5.4
通讯作者：
Goto SG
中科院分区：
文献类型：
--
作者：
Ikeno T;Tanaka SI;Numata H;Goto SG
大多数生物都进化出了生物钟，以预测每天的环境变化，其中许多生物也能够复杂地测量日照长度（光周期），用于调节季节性事件，如滞育，迁移和多态性。人们普遍认为，昼夜节律（每日）和季节节律（每年）都涉及相同的元素，因为两者都依赖于每日的明暗周期。然而，尽管这听起来很合理，但仍然没有确凿的证据表明昆虫中存在这种分子机制。我们已经通过在Riptortus pedestris中使用RNA干扰（RNAi）来解决这个问题。角质层的沉积节律表现出昼夜节律的主要特性，表明该节律是由生物钟调节的。针对周期和周期的生物钟基因（它们分别是生物钟中的负调节子和正调节子）的RNAi破坏了角质层沉积节律，并且这些RNAi产生了不同的角质层。同时，周期性RNAi导致昆虫在滞育诱导光周期下避免滞育，而周期性RNAi在滞育避免光周期下诱导滞育。保幼激素调控基因的表达模式和保幼激素类似物的应用表明，无论是卵巢发育本身还是保幼激素分泌的下游级联反应，都不会受到周期和周期RNAi的干扰。这项研究揭示了生物钟基因不仅对昼夜节律至关重要，而且对光周期滞育也至关重要。针对周期和周期的RNAi不仅在昼夜角质层沉积节律上有相反的作用，而且在光周期滞育中也有相反的作用。这些RNAi也对青少年表达调控基因的表达产生相反的影响。它仍然是可能的，生物钟基因多效性地影响卵巢发育，但基于这些结果，我们认为，生物钟基因，周期和周期，控制季节性的时间以及日常节奏的生物钟。参见评论：http://www.biomedcentral.com/1741-7007/8/115
Most organisms have evolved a circadian clock in order to anticipate daily environmental changes and many of these organisms are also capable of sophisticated measurement of daylength (photoperiodism) that is used to regulate seasonal events such as diapause, migration and polymorphism. It has been generally accepted that the same elements are involved in both circadian (daily) and seasonal (annual) rhythms because both rely upon daily light-dark cycles. However, as reasonable as this sounds, there remains no conclusive evidence of such a molecular machinery in insects. We have approached this issue by using RNA interference (RNAi) in Riptortus pedestris. The cuticle deposition rhythm exhibited the major properties of circadian rhythms, indicating that the rhythm is regulated by a circadian clock. RNAi directed against the circadian clock genes of period and cycle, which are negative and positive regulators in the circadian clock, respectively, disrupted the cuticle deposition rhythm and distinct cuticle layers were produced by these RNAi. Simultaneously, period RNAi caused the insect to avert diapause under a diapause-inducing photoperiod whereas cycle RNAi induced diapause under a diapause-averting photoperiod. The expression patterns of juvenile hormone-regulated genes and the application of juvenile hormone analogue suggested that neither ovarian development itself nor a downstream cascade of juvenile hormone secretion, were disturbed by period and cycle RNAi. This study revealed that the circadian clock genes are crucial not only for daily rhythms but also for photoperiodic diapause. RNAi directed against period and cycle had opposite effects not only in the circadian cuticle deposition rhythm but also in the photoperiodic diapause. These RNAi also had opposite effects on juvenile hormone-regulated gene expression. It is still possible that the circadian clock genes pleiotropically affect ovarian development but, based on these results, we suggest that the circadian clock operated by the circadian clock genes, period and cycle, governs seasonal timing as well as the daily rhythms. See Commentary: http://www.biomedcentral.com/1741-7007/8/115
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影响因子：
1.3
作者：
NUMATA, H;HIDAKA, T
通讯作者：
HIDAKA, T
影响因子：
3.5
作者：
Ikeno, Tornoko;Numata, Hideharu;Goto, Shin G.
通讯作者：
Goto, Shin G.
DOI：
10.1098/rstb.2001.0960
发表时间：
2001-11-29
影响因子：
6.3
作者：
Giebultowicz, JM
通讯作者：
Giebultowicz, JM
影响因子：
9.2
作者：
Collins, B;Mazzoni, EO;Blau, J
通讯作者：
Blau, J
DOI：
10.1007/bf00188108
发表时间：
1990-01-01
影响因子：
2.1
作者：
NUMATA, H
通讯作者：
NUMATA, H