Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response.
Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response.
复制标题
大鼠视网膜中受体相互作用蛋白3的差异神经元表达：参与缺血应激反应
DOI：
10.1186/1471-2202-14-16
复制
发表时间：
2013-02-02
期刊：
影响因子：
2.4
通讯作者：
Xiong K
中科院分区：
文献类型：
--
作者：
Huang JF;Shang L;Zhang MQ;Wang H;Chen D;Tong JB;Huang H;Yan XX;Zeng LP;Xiong K
受体相互作用蛋白3（Receptor-interacting protein 3，RIP 3）是RIP家族的成员，在细胞生物学研究中被证实参与程序性坏死或坏死性凋亡。有证据表明，坏死性凋亡可能是一种模式的神经元deathintheretina.ResultsIn本研究中，我们确定了RIP 3在正常大鼠视网膜的表达和急性高眼压（aHIOP）后的变化。RIP 3免疫反应性（IR）主要存在于视网膜内层，定位于神经节细胞层（GCL）和内核层（INL）中表达神经元特异性核抗原（NeuN）、小清蛋白和钙结合蛋白的细胞亚群。RIP 3与PKC-α和视紫红质均未发生双标记。RIP 3免疫反应性增加，在6小时和12小时的GCL，但减少在24小时的视网膜，没有明显的改变，在层或细胞分布模式。Western blot分析证实了RIP 3蛋白表达的上述时间依赖性变化。RIP 3表达细胞经常与碘化丙啶（PI）共定位。少数共定位细胞之间的RIP 3和Bax或切割caspase-3在GCL中观察aHIOP.ConclusionsThe结果表明，RIP 3差异表达在成年大鼠视网膜神经元，包括神经节细胞，无长突和水平细胞的子集。RIP 3蛋白水平在aHIOP后迅速升高。在aHIOP后，RIP 3标记与PI、Bax或裂解的caspase-3在神经节细胞层的细胞中共定位，这表明RIP 3参与了对急性缺血损伤的神经元反应。
BackgroundReceptor-interacting protein 3 (RIP3), a member of RIP family proteins, has been shown to participate in programmed necrosis or necroptosis in cell biology studies. Evidence suggests that necroptosis may be a mode of neuronal death in the retina.ResultsIn the present study we determined the expression of RIP3 in normal rat retina and its changes following acute high intraocular pressure (aHIOP). RIP3 immunoreactivity (IR) was largely present in the inner retinal layers, localized to subsets of cells expressing neuron-specific nuclear antigen (NeuN), parvalbumin and calbindin in the ganglion cell layer (GCL) and inner nuclear layer (INL). No double labeling was detected for RIP3 with PKC-α or rhodopsin. RIP3 immunoreactivity was increased in the GCL at 6 hr and 12 hr, but reduced at 24 hr in the retina, without apparent alteration in laminar or cellular distribution pattern. Western blot analysis confirmed the above time-dependent alteration in RIP3 protein expression. RIP3 expressing cells frequently co-localized with propidium iodide (PI). A few co-localized cells were observed between RIP3 and Bax or cleaved caspase-3 in the GCL in 12 hr following aHIOP.ConclusionsThe results indicate that RIP3 is expressed differentially in retinal neurons in adult rats, including subsets of ganglion cells, amacrine and horizontal cells. RIP3 protein levels are elevated rapidly following aHIOP. RIP3 labeling co-localized with PI, Bax or cleaved caspase-3 among cells in the ganglion cell layer following aHIOP, which suggest its involvement of RIP3 in neuronal responses to acute ischemic insults.
登录
查看更多内容
DOI：
10.3129/canjophthalmol.i07-036
发表时间：
2007-04-01
影响因子：
4.2
作者：
Nickells, Robert W.
通讯作者：
Nickells, Robert W.
影响因子：
4.8
作者：
Sun, XQ;Lee, J;Dixit, VM
通讯作者：
Dixit, VM
急性高眼压后大鼠视网膜局部血供的差异性变化与视网膜神经节细胞的选择性丢失有关
影响因子：
2
作者：
Tong, Jian-bin;Chen, Dan;Luo, Xue-gang
通讯作者：
Luo, Xue-gang
影响因子：
4
作者：
Fragoso, Miryam A.;Yi, Hyun;Hackam, Abigail S.
通讯作者：
Hackam, Abigail S.
影响因子：
3.5
作者：
Kasof, GM;Prosser, JC;Gomes, BC
通讯作者：
Gomes, BC