Microfluidic chip based micro RNA detection through the combination of fluorescence and surface enhanced Raman scattering techniques
Microfluidic chip based micro RNA detection through the combination of fluorescence and surface enhanced Raman scattering techniques
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基于微流控芯片的 micro RNA 检测，结合荧光和表面增强拉曼散射技术
DOI：
10.1088/1361-6528/aa527b
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发表时间：
2017-01
期刊：
影响因子：
3.5
通讯作者：
Yiping Cui2
中科院分区：
文献类型：
--
作者：
Zhile Wang;Shenfei Zong;Zhuyuan Wang;Lei Wu;Peng Chen;Binfeng Yun;Yiping Cui2
提出了一种基于微流控芯片的荧光和表面增强拉曼散射（Sers）相结合的检测microRNA（miRNA）的新方法。首先，将银纳米颗粒（Ag NPs）固定在载玻片上，形成Sers增强基底。然后将专门设计的分子信标（MB）附着到Sers基底上。MB的3′端用巯基修饰以促进MB的附着，而MB的5′端用有机染料6-FAM标记，其用作荧光团和Sers报告子。在不存在靶miRNA的情况下，MB将形成发夹结构，使得6-FAM接近Ag NP。因此，6-FAM的荧光将被猝灭，6-FAM的拉曼信号将被增强。相反，在靶miRNA存在的情况下，miRNA和MB之间的杂交将解折叠MB并增加6-FAM和Ag NP之间的距离。因此，6-FAM的荧光将恢复，6-FAM的Sers信号将降低。因此，目标miRNA将同时引入荧光和Sers信号强度的相反变化趋势。通过组合两个光谱中的相反变化，与单独使用荧光或Sers相比，实现了对靶miRNA的改善的灵敏度和线性。此外，微流控芯片的引入可以减少反应时间、试剂用量和检测的复杂性。该方法具有灵敏度高、操作简便等优点，有望在miRNA相关疾病的研究中发挥重要作用。
We present a novel microfluidic chip based method for the detection of micro RNA (miRNA) via the combination of fluorescence and surface enhanced Raman scattering (SERS) spectroscopies. First, silver nanoparticles (Ag NPs) are immobilized onto a glass slide, forming a SERS enhancing substrate. Then a specificially designed molecular beacon (MB) is attached to the SERS substrate. The 3′ end of the MB is decorated with a thiol group to facilitate the attachment of the MB, while the 5′ end of the MB is labeled with an organic dye 6-FAM, which is used both as the fluorophore and SERS reporter. In the absence of target miRNA, the MB will form a hairpin structure, making 6-FAM close to the Ag NPs. Hence, the fluorescence of 6-FAM will be quenched and the Raman signal of 6-FAM will be enhanced. On the contrary, with target miRNA present, hybridization between the miRNA and MB will unfold the MB and increase the distance between 6-FAM and the Ag NPs. Thus the fluorescence of 6-FAM will recover and the SERS signal of 6-FAM will decrease. So the target miRNA will simultaneously introduce opposite changing trends in the intensities of the fluorescence and SERS signals. By combining the opposite changes in the two optical spectra, an improved sensitivity and linearity toward the target miRNA is achieved as compared with using solely fluorescence or SERS. Moreover, introducing the microfluidic chip can reduce the reaction time, reagent dosage and complexity of detection. With the improved sensitivity and simplicity, we anticipate that the presented method can have great potential in the investigation of miRNA related diseases.
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影响因子：
7.4
作者：
D. van Lierop;I. A. Larmour;K. Faulds;D. Graham
通讯作者：
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DOI：
10.1038/nrc3932
发表时间：
2015-06
期刊：
Nature reviews. Cancer
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
12.6
作者：
Wang, Zhuyuan;Zong, Shenfei;Cui, Yiping
通讯作者：
Cui, Yiping
影响因子：
3.3
作者：
M. Zharnikov;S. Frey;Haoxiang Rong;Y.J. Yang;K. Heister;M. Buck;M. Grunze
通讯作者：
M. Zharnikov;S. Frey;Haoxiang Rong;Y.J. Yang;K. Heister;M. Buck;M. Grunze