Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling.
Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling.
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DOI：
10.1016/j.synbio.2018.01.001
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发表时间：
2018-03
影响因子：
4.8
通讯作者：
Polizzi KM
中科院分区：
文献类型：
--
作者：
Jonas FRH;Royle KE;Aw R;Stan GV;Polizzi KM
适应使生物体能够维持恒定的内部环境，从而优化生长。未折叠蛋白反应（UPR）是维持内质网（ER）稳态的反馈环路的一个例子，并且是转录网络如何介导适应的特征。然而，最近发现的维持内质网稳态的不对称分裂是替代非转录途径如何存在的一个例子，但却被金标准转录组或基于蛋白质组群体的检测所忽视。在这项研究中，我们结合使用荧光报告基因、流式细胞术和数学模型来探索不对称细胞分裂和 UPR 在维持 ER 稳态中的相对作用。在低内质网应激下，不对称分裂使子细胞缺乏内质网，需要激活 UPR 并延长细胞周期，在此期间它们可以在生长前恢复内质网功能。我们的数学模型的数学分析和模拟结果强化了实验观察，即低内质网压力主要影响子细胞的生长速率。这些结果证明了稳态途径之间的相互作用以及探索亚种群动态以了解种群对定量不同压力的适应的重要性。
Adaptation allows organisms to maintain a constant internal environment, which is optimised for growth. The unfolded protein response (UPR) is an example of a feedback loop that maintains endoplasmic reticulum (ER) homeostasis, and is characteristic of how adaptation is often mediated by transcriptional networks. The more recent discovery of asymmetric division in maintaining ER homeostasis, however, is an example of how alternative non-transcriptional pathways can exist, but are overlooked by gold standard transcriptomic or proteomic population-based assays. In this study, we have used a combination of fluorescent reporters, flow cytometry and mathematical modelling to explore the relative roles of asymmetric cell division and the UPR in maintaining ER homeostasis. Under low ER stress, asymmetric division leaves daughter cells with an ER deficiency, necessitating activation of the UPR and prolonged cell cycle during which they can recover ER functionality before growth. Mathematical analysis of and simulation results from our mathematical model reinforce the experimental observations that low ER stress primarily impacts the growth rate of the daughter cells. These results demonstrate the interplay between homeostatic pathways and the importance of exploring sub-population dynamics to understand population adaptation to quantitatively different stresses.
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影响因子：
7.7
作者：
Carrara M;Prischi F;Nowak PR;Kopp MC;Ali MM
通讯作者：
Ali MM
影响因子：
2.6
作者：
Houser, John R.;Ford, Eintou;Errede, Beverly
通讯作者：
Errede, Beverly
DOI：
10.1098/rspb.1964.0064
发表时间：
1964-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HASSELBACH, W
通讯作者：
HASSELBACH, W
影响因子：
2.1
作者：
Ogawa, N;Mori, K
通讯作者：
Mori, K
DOI：
10.1039/c2mt00185c
发表时间：
2012-07
期刊：
Metallomics : integrated biometal science
影响因子：
--
作者：
Martinez-Finley EJ;Chakraborty S;Fretham SJ;Aschner M
通讯作者：
Aschner M