The THO/TREX Complex Component RAE2/TEX1 Is Involved in the Regulation of Aluminum Resistance and Low Phosphate Response in Arabidopsis.
The THO/TREX Complex Component RAE2/TEX1 Is Involved in the Regulation of Aluminum Resistance and Low Phosphate Response in Arabidopsis.
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THO/TREX 复合物组分 RAE2/TEX1 参与拟南芥耐铝性和低磷酸盐响应的调节
DOI：
10.3389/fpls.2021.698443
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发表时间：
2021
影响因子：
5.6
通讯作者：
Huang CF
中科院分区：
文献类型：
--
作者：
Zhu YF;Guo J;Zhang Y;Huang CF
C2H2型锌指转录因子对质子RHIZOTOXICY1(STOP1)敏感，主要通过促进苹果酸转运蛋白编码基因拟南芥铝激活的苹果酸转运蛋白1(AtALMT1)的表达，在铝(Al)抗性和低磷(PI)反应中发挥关键作用。我们以前的研究表明，ATALMT1表达3(RAE3/HPR1)的调节是THO/TREX复合体的核心成分，它参与了核质STOP1 mRNA输出的调节，从而调节了铝抗性和低PI反应。在此，我们报道了THO复合体的另一个核心成分RAE2/TEX1也参与了铝抗性和低等电点响应的调节。RAE2的突变降低了STOP1下游基因的表达，包括AtALMT1。Rae2对铝的敏感性低于rae3，这与rae3根的苹果酸分泌量低于rae2根的情况一致。然而，RAE2的低PI反应比RAE3更严重，这表明RAE2也调节ATALMT1非依赖的途径来调节低PI反应。此外，与调节STOP1mRNA输出的RAE3不同，突变的RAE2不影响STOP1mRNA在细胞核中的积累，尽管RAE2中STOP1的蛋白水平降低。在rae2突变背景中引入rae1突变可以部分恢复rae2的缺陷表型。综上所述，我们的结果表明，RAE2和RAE3在铝抗性和低等电点响应的调节中发挥着重叠但不同的作用。
The C2H2-type zinc finger transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) plays a critical role in aluminum (Al) resistance and low phosphate (Pi) response mainly through promoting the expression of the malate transporter-encoding gene ARABIDOPSIS THALIANA ALUMINUM ACTIVATED MALATE TRANSPORTER 1 (AtALMT1). We previously showed that REGULATION OF ATALMT1 EXPRESSION 3 (RAE3/HPR1), a core component of the THO/TREX complex, is involved in the regulation of nucleocytoplasmic STOP1 mRNA export to modulate Al resistance and low Pi response. Here, we report that RAE2/TEX1, another core component of the THO complex, is also involved in the regulation of Al resistance and low Pi response. Mutation of RAE2 reduced the expression of STOP1-downstream genes, including AtALMT1. rae2 was less sensitive to Al than rae3, which was consistent with less amount of malate secreted from rae3 roots than from rae2 roots. Nevertheless, low Pi response was impaired more in rae2 than in rae3, suggesting that RAE2 also regulates AtALMT1-independent pathway to modulate low Pi response. Furthermore, unlike RAE3 that regulates STOP1 mRNA export, mutating RAE2 did not affect STOP1 mRNA accumulation in the nucleus, although STOP1 protein level was reduced in rae2. Introduction of rae1 mutation into rae2 mutant background could partially recover the deficient phenotypes of rae2. Together, our results demonstrate that RAE2 and RAE3 play overlapping but distinct roles in the modulation of Al resistance and low Pi response.
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影响因子：
16
作者：
Johnson, Sara Ann;Cubberley, Gabrielle;Bentley, David L.
通讯作者：
Bentley, David L.
影响因子：
14.9
作者：
Gromadzka AM;Steckelberg AL;Singh KK;Hofmann K;Gehring NH
通讯作者：
Gehring NH
影响因子：
64.5
作者：
Cheng, Hong;Dufu, Kobina;Reed, Robin
通讯作者：
Reed, Robin
影响因子：
14.8
作者：
Folta, Kevin M.;Kaufman, Lon S.
通讯作者：
Kaufman, Lon S.
DOI：
10.1073/pnas.0700117104
发表时间：
2007-06-05
影响因子：
11.1
作者：
Iuchi, Satoshi;Koyama, Hiroyuki;Kobayashi, Masatomo
通讯作者：
Kobayashi, Masatomo