Widespread distribution of resistance to triazole fungicides in Brazilian populations of the wheat blast pathogen
Widespread distribution of resistance to triazole fungicides in Brazilian populations of the wheat blast pathogen
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巴西麦瘟病原菌群体对三唑类杀菌剂的抗性广泛分布
DOI：
10.1111/ppa.13288
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发表时间：
2020
期刊：
影响因子：
2.7
通讯作者：
Poloni N
中科院分区：
文献类型：
--
作者：
Poloni N
在巴西，杀真菌剂在控制小麦稻瘟病方面没有效果。对2012年期间从巴西中南部6个种群的麦田中取样的179个Pyricularia Triticumlineage（PoTl）菌株的早期分析发现了对嗜球果伞素杀菌剂的高水平抗性。在这里，我们分析了唑类药物耐药性在相同的菌株戊唑醇和氟环唑的EC 50测量的基础上。所有6个巴西种群的PoTl对两种唑类都表现出高抗性，体外EC 50值至少比推荐的田间剂量高35至50倍。我们对CYP51A和CYP51B基因进行测序，以确定它们是否可能在观察到的唑类耐药中发挥作用。虽然我们在PoT1中发现了5种不同的单倍型，其中4种在CYP51A中进行了非同义替换，但这些替换均与EC 50升高无关。PoT1CYP51 A和CYP51 B均能补充酵母CYP51的功能。所有表达PoTlCYP 51A的酵母转化体对三唑的敏感性低于PoTlCYP 51Bones。表达携带R158K取代的PoTlCYP 51 A单倍型H1的转化体并不比表达PoTlCYP 51 A单倍型H5的转化体更耐药，所述单倍型H5与在来自稻瘟病的三唑敏感的稻瘟病菌分离株中发现的单倍型H6同义。因此，与稻瘟病分离物相比，小麦稻瘟病分离物的三唑敏感性降低似乎与在更高暴露于三唑后获得的非靶位点相关的抗性机制有关。
Fungicides have not been effective in controlling the wheat blast disease in Brazil. An earlier analysis of 179 isolates ofPyricularia oryzae Triticumlineage (PoTl) sampled from wheat fields across six populations in central‐southern Brazil during 2012 discovered a high level of resistance to strobilurin fungicides. Here we analysed azole resistance in the same strains based on EC50measurements for tebuconazole and epoxiconazole. All six Brazilian populations of PoTl exhibited high resistance to both azoles, with in vitro EC50values that were at least 35 to 50 times higher than the recommended field doses. We sequenced theCYP51AandCYP51Bgenes to determine if they were likely to play a role in the observed azole resistance. Although we found five distinct haplotypes in PoTl carrying four nonsynonymous substitutions inCYP51A, none of these substitutions were correlated with elevated EC50.CYP51Bwas sequenced for nine PoTl isolates, three each representing low, medium, and high tebuconazole EC50. Both PoTlCYP51AandCYP51Bcould complement yeastCYP51function. All PoTlCYP51A‐expressing yeast transformants were less sensitive to triazoles than the PoTlCYP51Bones. Transformants expressing PoTlCYP51Ahaplotype H1 carrying the R158K substitution were not more resistant than those expressing PoTlCYP51Ahaplotype H5, which is synonymous to haplotype H6, found in triazole‐sensitiveP.oryzae Oryzaisolates from rice blast. Therefore, the reduced triazole sensitivity of wheat blast isolates compared to rice blast isolates appears to be associated with a non‐target‐site related resistance mechanism acquired after higher exposure to triazoles.
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控制 QUÍMICO DA FERRUGEM DA FOLHA EM CULTIVARES DE TRIGO SUBMETIDAS A DIFERENTES NÍVEIS DE ADUBAÇÍNITROGENADA
DOI：
--
发表时间：
2009
期刊：
影响因子：
--
作者：
D. Debona;Diego Dalla Favera;G. D. Corte;L. D. S. Domingues;Ricardo Silveiro Balardin
通讯作者：
Ricardo Silveiro Balardin
影响因子：
2.7
作者：
Cruz, C. D.;Kiyuna, J.;Valent, B.
通讯作者：
Valent, B.
影响因子：
--
作者：
J. Rocha;Adérico Júnior Badaró Pimentel;Guilherme Ribeiro;Moacil Alves de Souza
通讯作者：
Moacil Alves de Souza
使用合成杀菌剂、部分抗性以及硅酸盐和亚磷酸盐矿物质防治小麦瘟病
影响因子：
1.4
作者：
A. Pagani;A. C. Dianese;A. Café
通讯作者：
A. Café
巴西小麦稻瘟病菌的寄主范围、交配类型和繁殖力
DOI：
--
发表时间：
1993
期刊：
影响因子：
--
作者：
A. S. Urashima;S. Igarashi;H. Kato
通讯作者：
H. Kato