Effect of the pollution level on the functional bacterial groups aiming at degrading bisphenol A and nonylphenol in natural biofilms of an urban river
Effect of the pollution level on the functional bacterial groups aiming at degrading bisphenol A and nonylphenol in natural biofilms of an urban river
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污染水平对城市河流天然生物膜中双酚A和壬基酚降解功能菌群的影响
DOI：
10.1007/s11356-016-6757-3
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发表时间：
2016-05
影响因子：
5.8
通讯作者：
Wang Chao
中科院分区：
文献类型：
--
作者：
Cai Wei;Li Yi;Wang Peifang;Niu Lihua;Zhang Wenlong;Wang Chao
双酚A(BPA)和4-壬基酚(NP)是水环境中普遍存在的具有雌激素活性的污染物，因其对内分泌系统的干扰而引起全球关注。采用基于16S rRNA基因序列的末端限制性片段长度多态性方法，研究了城市河流生物膜中参与降解BPA和NP的细菌群的空间分布特征。还评估了污染水平和水质参数对这些群体的影响。根据水质数据和中国《地表水环境质量标准》(GB3838-2002)，用系统聚类法将采样点划分为3类，反映了各采样点营养盐相对轻度污染(SP)、中度污染(MP)和重度污染(HP)的不同程度。L−1河水中双酚A和NP的浓度分别为0.8~77.5 ng和10.2~162.9 ng，分别占总序列的9.8%、8.1%、7.6%、6.7%、6.2%、4.1%和2.8%。这些类群的总丰度呈现出轻微上升的趋势，随后随着污染程度的增加而迅速下降。MP河段产气单胞菌的平均比例是SP或HP河段的1.5-2倍。官能团的分布与环境变量有关，特别是pH、电导率、氨氮(NH3-N)和双酚A。芽孢杆菌和根瘤菌的丰度与较高的pH值和电导率以及较低的NP和BPA浓度密切相关；产碱菌和假单胞菌与较高的TP和CODMn浓度相关，与DO浓度呈负相关。本研究可为城市污染河流的细菌群变化提供有效的数据。
Bisphenol A (BPA) and 4-nonylphenol (NP) are ubiquitous pollutants with estrogenic activity in aquatic environment and have attracted global concern due to their disruption of endocrine systems. This study investigated the spatial distribution characteristics of the bacterial groups involved in the degradation of BPA and NP within biofilms in an urban river using terminal restriction fragment length polymorphism based on 16S rRNA gene sequences. The effects of the pollution level and water parameters on these groups were also assessed. Hierarchical cluster analysis grouped the sampling sites into three clusters reflecting their varying nutrient pollution levels of relatively slight pollution (SP), moderate pollution (MP), and high pollution (HP) based on water quality data and Environmental Quality Standard for Surface Water of China (GB3838-2002). The BPA and NP concentration in river water ranged from 0.8 to 77.5 and 10.2 to 162.9 ng L−1, respectively.Comamonadaceae,Pseudomonadaceae,Alcaligenaceae,Bacillaceae,Sphingomonadacea,Burkholderiaceae, andRhizobiaceaewere the dominant bacterial taxa involved in BPA and NP degradation, comprising an average of 9.8, 8.1, 7.6, 6.7, 6.2, 4.1, and 2.8 % of total sequences, respectively. The total abundance of these groups showed a slight upward trend and subsequently rapidly decreased with increasing pollution levels. The average proportion ofComamonadaceaein MP river sections was almost 1.5–2 times than that in SP or HP one. The distribution of functional groups was found related to environmental variables, especially pH, conductivity, ammonium nitrogen (NH3-N), and BPA. The abundance ofComamonadaceaeandRhizobiaceaewas both closely related to higher values of pH and conductivity as well as lower concentrations of NP and BPA.AlcaligenaceaeandPseudomonadaceaewere associated with higher concentrations of TP and CODMnand inversely correlated with DO concentration. This study might provide effective data on bacterial group changes in polluted urban rivers.
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影响因子：
2.4
作者：
Minghua Zhang;Jianming Xu
通讯作者：
Minghua Zhang;Jianming Xu
影响因子：
2.7
作者：
M. Chadwick
通讯作者：
M. Chadwick
影响因子：
5.8
作者：
Zhao Wang;Yuyin Yang;Weimin Sun;Yu Dai;Shuguang Xie
通讯作者：
Zhao Wang;Yuyin Yang;Weimin Sun;Yu Dai;Shuguang Xie
影响因子：
1.9
作者：
K. Fujii;N. Urano;S. Kimura;Y. Nomura;I. Karube
通讯作者：
K. Fujii;N. Urano;S. Kimura;Y. Nomura;I. Karube
影响因子：
4.1
作者：
Lijun Nie;Li-hong Wang;Qingqing Wang;Shengman Wang;Qing Zhou;Xiaohua Huang
通讯作者：
Lijun Nie;Li-hong Wang;Qingqing Wang;Shengman Wang;Qing Zhou;Xiaohua Huang