Photosynthetic efficiency, cell volume, and elemental stoichiometric ratios in Thalassirosira weissflogii under phosphorus limitation
Photosynthetic efficiency, cell volume, and elemental stoichiometric ratios in Thalassirosira weissflogii under phosphorus limitation
复制标题
磷限制下魏氏海链藻的光合效率、细胞体积和元素化学计量比
DOI：
10.1007/s00343-011-0224-2
复制
发表时间：
2011-08
影响因子：
--
通讯作者：
Lin Senjie
中科院分区：
文献类型：
--
作者：
Liu Sheng;Guo Zhiling;Li Tao;Huang Hui;Lin Senjie
已知营养盐限制会抑制浮游植物的生长和代谢，并改变浮游植物中元素的化学计量比。在这项研究中，在海链藻weissflorus的生理变化进行了测量下，不同的溶解无机磷酸盐（DIP）制度在半连续培养，重新评估的效用Redfield比率的营养限制。结果表明，随着DIP可用性的降低，泡孔尺寸增大。缺磷处理（f/2-P）的细胞大小是磷限制处理（f/100）的1.48倍，是磷饱和处理（f/2和f/10）的2.67倍。岩藻黄素与叶绿素a的比值（Fuco/chla）在磷饱和培养中相对稳定（约0.3），比磷限制培养和缺磷培养高10倍。在试验期间，磷饱和培养条件下，光合效率指数ΔF/Fm′相对稳定在0.50左右，但随着DIP利用率的降低，ΔF/Fm ′迅速下降。尽管磷饱和培养和磷限制培养的细胞磷含量（1.6pg/cell和0.7pg/cell）有显著差异，但T. weissfloccult没有显示出与DIP可用性的趋势，并在25附近略有波动。我们的研究结果表明，T。weissflocculant不是严格的大小门控，但可能是由生物化学，因此，元素的化学计量比阈值，和细胞的N/P比从Redfield比的偏差是不是一个可靠的指标藻类营养胁迫。
Nutrient limitation is known to inhibit growth and metabolism and to alter elemental stoichiometric ratios in phytoplankton. In this study, physiological changes in Thalassirosira weissflogii were measured under different dissolved inorganic phosphate (DIP) regimes in semi-continuous cultures to revisit the utility of the Redfield ratio for assessing nutrient limitation. The results showed that cell size increased with decreasing DIP availability. In the P-depleted treatment (f/2-P) the cell size was 1.48 times larger than that in the P-limited (f/100) treatment and 2.67 times larger than that in the P-saturated treatment (f/2 and f/10). The fucoxanthin to chlorophyll a ratio (Fuco/chl a) was relatively stable (about 0.3) in P-saturated cultures and was 10 times higher than that in P-limited and P-depleted cultures. During the experimental period, the photosynthetic efficiency index, ΔF/Fm′, was relatively stable at ∼0.50 in the P-saturated cultures, but quickly declined with decreasing DIP availability. Although cellular P content showed a significant difference between the P-saturated culture (1.6 pg/cell) and the P-limited culture (0.7 pg/cell), the N/P ratio in T. weissflogii did not show a trend with DIP availability and fluctuated slightly around 25. Our results suggest that cell division in T. weissflogii is not strictly size-gated but is probably regulated by a biochemical, and hence, an elemental stoichiometric ratio threshold, and that deviation of the cellular N/P ratio from the Redfield ratio is not a reliable indicator of algal nutrient stress.
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影响因子：
2.1
作者：
A. C. Redfield
通讯作者：
A. C. Redfield
DOI：
--
发表时间：
2008
期刊：
--
影响因子：
--
作者：
Yuan-Yuan Liang-Yuan
通讯作者：
Yuan-Yuan Liang-Yuan
影响因子：
2.1
作者：
Sun, J;Liu, DY
通讯作者：
Liu, DY
影响因子：
2.1
作者：
F. Vidussi;H. Claustre;J. Bustillos-Guzmán;C. Cailliau;J. Marty
通讯作者：
F. Vidussi;H. Claustre;J. Bustillos-Guzmán;C. Cailliau;J. Marty
影响因子：
64.8
作者：
Tyrrell, T
通讯作者：
Tyrrell, T