Laminin gamma2 fragments are increased in the circulation of patients with early phase acute lung injury.
Laminin gamma2 fragments are increased in the circulation of patients with early phase acute lung injury.
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DOI：
10.1007/s00134-009-1719-6
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发表时间：
2010-03
影响因子：
38.9
通讯作者：
Hashimoto, Satoru
中科院分区：
文献类型：
--
作者：
Katayama, Masahiko;Ishizaka, Akitoshi;Sakamoto, Michiie;Fujishima, Seitaro;Sekiguchi, Kiyotoshi;Asano, Koichiro;Betsuyaku, Tomoko;Kotani, Toru;Ware, Lorraine B.;Matthay, Michael A.;Hashimoto, Satoru
层粘连蛋白-5是一种仅由上皮细胞表达的细胞黏附分子，在其基本成分γ2-链被蛋白质降解后，被认为可以促进上皮细胞的迁移和损伤上皮的修复。我们先前的研究表明，循环中层粘连蛋白γ2链氨基末端片段的水平反映了癌症患者上皮性肿瘤的侵袭性，但其在肺泡上皮损伤中的生理作用尚不清楚。对急性肺损伤(ALI)或相关疾病患者的上皮内衬液或肺水肿液进行采样。血浆样本是在发病时或以后从他们那里获得的。用自制的免疫测定法测定G2F浓度。我们发现ALI患者肺水肿和上皮衬里液中G2F的含量明显高于其他呼吸系统疾病患者。与心源性肺水肿组(59±0.36)和特发性肺纤维化组(37±0.17)相比，ALI患者发病早期血浆LN-5水平显著升高(P<0.05)，提示ALI患者肺泡上皮细胞迅速分泌层粘连蛋白-5。在发病后5天，死亡患者维持较高的血浆浓度(152±0.84)，而存活患者的血浆浓度下降(71±0.35)，提示层粘连蛋白-5的分泌受到抑制，与ALI的恢复有关。循环G2F可能是ALI发病早期分泌的肺泡层粘连蛋白-5的生物标志物，潜在地调节肺泡的再上皮化。
Laminin-5, a cell adhesive molecule expressed solely by epithelium, is known to enhance epithelial cell migration and repair of injured epithelium, after its essential component γ2-chain is processed proteolytically. Our previous study revealed circulating levels of amino-terminal fragment of laminin γ2-chain (G2F) reflect epithelial tumor invasiveness in carcinoma patients, but its physiological role in alveolar epithelial injury remains unknown. Sampling of epithelial lining fluids or pulmonary edema fluids from patients with acute lung injury (ALI) or related diseases was performed. Plasma samples were obtained from them at the time of disease onset or later. G2F concentrations were determined by immunoassay constructed by ourselves. We found a significantly higher amount of G2F in pulmonary edema and epithelial lining fluids of patients with ALI, as compared with those with the other respiratory diseases. Their plasma levels were also elevated significantly early at the onset of ALI (mean ± SD; 147 ± 82 ng/ml in non-surviving and 90 ± 56 in surviving patients) as compared with those in the patients with cardiogenic pulmonary edema (59 ± 36) or idiopathic pulmonary fibrosis (37 ± 17), indicating alveolar epithelium rapidly secrete laminin-5 in ALI. At 5 days after onset, non-surviving patients maintained higher plasma concentrations (152 ± 84), but in contrast, the levels in surviving patients declined (71 ± 35), suggesting secretion of laminin-5 was suppressed, associated with recovery from ALI. Circulating G2F may be a biomarker for alveolar laminin-5 secreted early at disease onset in ALI, potentially regulating alveolar re-epithelialization.
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DOI：
10.1083/jcb.114.3.567
发表时间：
1991-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Rousselle P;Lunstrum GP;Keene DR;Burgeson RE
通讯作者：
Burgeson RE
DOI：
10.1164/ajrccm/144.2.452
发表时间：
1991-08-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
LWEBUGAMUKASA, JS
通讯作者：
LWEBUGAMUKASA, JS
DOI：
10.1164/rccm.200208-966ws
发表时间：
2003-04-01
影响因子：
24.7
作者：
Matthay, MA;Zimmerman, GA;Harabin, AL
通讯作者：
Harabin, AL
DOI：
10.1164/ajrccm/142.6_pt_1.1250
发表时间：
1990-12-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
MATTHAY, MA;WIENERKRONISH, JP
通讯作者：
WIENERKRONISH, JP
DOI：
10.1165/ajrcmb.19.2.3087
发表时间：
1998-08-01
影响因子：
6.4
作者：
Pierce, RA;Griffin, GL;Senior, RM
通讯作者：
Senior, RM