The vital hormone 20-hydroxyecdysone controls ATP production by upregulating binding of trehalase 1 with ATP synthase subunit α in Helicoverpa armigera.
The vital hormone 20-hydroxyecdysone controls ATP production by upregulating binding of trehalase 1 with ATP synthase subunit α in Helicoverpa armigera.
复制标题
重要激素 20-羟基蜕皮酮通过上调棉铃虫中海藻糖酶 1 与 ATP 合成酶亚基 α 的结合来控制 ATP 产生
DOI：
10.1016/j.jbc.2022.101565
复制
发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Zhao W
中科院分区：
文献类型：
--
作者：
Chang Y;Zhang B;Du M;Geng Z;Wei J;Guan R;An S;Zhao W
海藻糖是昆虫的主要“血糖”，在能量供应和应激保护中起着至关重要的作用。海藻糖的降解只能在海藻糖酶(Treh)的酶控制下进行，海藻糖酶在生长发育、能量供应、甲壳素的生物合成和非生物胁迫反应中起着重要的作用。以往的报道表明，重要的激素20-羟基蜕皮激素(20E)调节Treh，但20E调节Treh的具体机制尚不清楚。在本研究中，我们研究了HaTreh1在棉铃虫幼虫中的功能。结果表明，在蜕皮和变态过程中，HaTreh1的转录水平和酶活性均在蜕皮、中肠和脂肪体中升高，20E通过经典的核受体复合体ECR-B1/USP1上调HaTreh1的转录水平。HaTreh1是一种线粒体蛋白。我们还发现，在五龄或六龄幼虫中敲除HaTreh1会导致体重减轻和死亡率增加。酵母双杂交、免疫共沉淀和谷胱甘肽-S-转移酶(GST)下拉实验表明，HaTreh1与三磷酸腺苷合成酶亚单位α(HaATPS-α)结合，并且这种结合在20E处理下增加。此外，20E还提高了HAATPS-α的转录水平和含量。最后，HaTreh1或HaATPS-α基因的敲除降低了20E对ATP含量的诱导效应。综上所述，这些发现表明，20E通过上调HaTreh1与HaATPS-α的结合来控制三磷酸腺苷的产生。
Trehalose is the major “blood sugar” of insects and it plays a crucial role in energy supply and as a stress protectant. The hydrolysis of trehalose occurs only under the enzymatic control of trehalase (Treh), which plays important roles in growth and development, energy supply, chitin biosynthesis, and abiotic stress responses. Previous reports have revealed that the vital hormone 20-hydroxyecdysone (20E) regulates Treh, but the detailed mechanism underlying 20E regulating Treh remains unclear. In this study, we investigated the function of HaTreh1 in Helicoverpa armigera larvae. The results showed that the transcript levels and enzymatic activity of HaTreh1 were elevated during molting and metamorphosis stages in the epidermis, midgut, and fat body, and that 20E upregulated the transcript levels of HaTreh1 through the classical nuclear receptor complex EcR-B1/USP1. HaTreh1 is a mitochondria protein. We also found that knockdown of HaTreh1 in the fifth- or sixth-instar larvae resulted in weight loss and increased mortality. Yeast two-hybrid, coimmunoprecipitation, and glutathione-S-transferase (GST) pull-down experiments demonstrated that HaTreh1 bound with ATP synthase subunit alpha (HaATPs-α) and that this binding increased under 20E treatment. In addition, 20E enhanced the transcript level of HaATPs-α and ATP content. Finally, the knockdown of HaTreh1 or HaATPs-α decreased the induction effect of 20E on ATP content. Altogether, these findings demonstrate that 20E controls ATP production by up-regulating the binding of HaTreh1 to HaATPs-α in H. armigera.
登录
查看更多内容
影响因子：
56.9
作者：
CLEGG, JS;EVANS, DR
通讯作者：
EVANS, DR
影响因子：
2.2
作者：
Kamei, Yuhki;Hasegawa, Yuh;Yaginuma, Toshinobu
通讯作者：
Yaginuma, Toshinobu
影响因子：
3.2
作者：
CHANG, PL;MORRISON, PE
通讯作者：
MORRISON, PE
影响因子：
2.2
作者：
Gu, Jianhua;Shao, Ying;Zhang, Yongjun
通讯作者：
Zhang, Yongjun
DOI：
10.1007/bf01919312
发表时间：
1996-05-01
期刊：
EXPERIENTIA
影响因子：
--
作者：
Becker, A;Schloder, P;Wegener, G
通讯作者：
Wegener, G